Protein Info for GFF2462 in Xanthobacter sp. DMC5
Annotation: Gamma-glutamylputrescine synthetase PuuA
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K01915, glutamine synthetase [EC: 6.3.1.2] (inferred from 96% identity to xau:Xaut_1939)Predicted SEED Role
"glutamine synthetase family protein" in subsystem Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis
MetaCyc Pathways
- L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- superpathway of ammonia assimilation (plants) (3/3 steps found)
- ammonia assimilation cycle I (2/2 steps found)
- ammonia assimilation cycle II (2/2 steps found)
- L-glutamine biosynthesis I (1/1 steps found)
- L-glutamate and L-glutamine biosynthesis (5/7 steps found)
- L-aspartate degradation II (aerobic) (1/3 steps found)
- L-aspartate degradation III (anaerobic) (1/3 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.3.1.2
Use Curated BLAST to search for 6.3.1.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (467 amino acids)
>GFF2462 Gamma-glutamylputrescine synthetase PuuA (Xanthobacter sp. DMC5) MAKRSKGAQKAQATAPRGVTSLTEAKAWMASRGITEIECTVPDLAGVARGKIMPASKFFS SPVMNLPLSVFFQTISGEYPDYDGLVDSVIADSDLVLEPDLATLCSVPWAQDPTAQVIHD AYHRDGRPVELAPRQVLRHVISLYEKKGWKPVVAPEIEFYLVEPNTDPDYPLKPPVGRSG RPEIGRQSYSIQAVNEFDALFEDIYDYSEAQGLEIDTLIHEDGAAQMEINLRHGDPLVLA DQVFLFKRTIREAALAHKIYATFMAKPIANEPGSAMHIHQSVINTATGRNLFSDEDGDPT PEFFSFIAGHQKYLPSVMCIMAPYVNSYRRLTRDSMAPINLQWGYDNRTAGLRVPPSTPE ARRLENRVPSSDANPYLAIAASLACGYLGLTENLRATDPIDGDAKNLDFDLPRGLLEAVA AFQDSEPLAEVLGERFVSAYAAVKQAEFETFMRVISPWEREYLLLNV