Protein Info for GFF2459 in Variovorax sp. SCN45

Annotation: CzcABC family efflux RND transporter, transmembrane protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1043 transmembrane" amino acids 12 to 29 (18 residues), see Phobius details amino acids 334 to 352 (19 residues), see Phobius details amino acids 359 to 379 (21 residues), see Phobius details amino acids 386 to 408 (23 residues), see Phobius details amino acids 433 to 453 (21 residues), see Phobius details amino acids 465 to 491 (27 residues), see Phobius details amino acids 523 to 542 (20 residues), see Phobius details amino acids 858 to 877 (20 residues), see Phobius details amino acids 885 to 904 (20 residues), see Phobius details amino acids 911 to 933 (23 residues), see Phobius details amino acids 961 to 981 (21 residues), see Phobius details amino acids 989 to 1015 (27 residues), see Phobius details TIGR00914: heavy metal efflux pump, CzcA family" amino acids 1 to 1025 (1025 residues), 919.2 bits, see alignment E=1.6e-280 PF00873: ACR_tran" amino acids 8 to 1015 (1008 residues), 817.8 bits, see alignment E=1.3e-249 PF03176: MMPL" amino acids 326 to 493 (168 residues), 33.1 bits, see alignment E=3e-12 amino acids 856 to 1027 (172 residues), 24.2 bits, see alignment E=1.5e-09

Best Hits

KEGG orthology group: K07239, heavy-metal exporter, HME family (inferred from 74% identity to dia:Dtpsy_3517)

Predicted SEED Role

"Cobalt-zinc-cadmium resistance protein CzcA; Cation efflux system protein CusA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1043 amino acids)

>GFF2459 CzcABC family efflux RND transporter, transmembrane protein (Variovorax sp. SCN45)
MIERLVTLCFHRRGIAWIVFAFVALYGWTCWKQLPIEAYPDIADVTSQVVTQVPGLAAEE
VEQQITVPLERELLSTPGLHVLRSKSTFGLSLIVVVFKDGVEGYWSRQRLQERISGVTLP
HGATPGLDAYTSPIGEIYRYTLESKTRDLRELSELQSWTVIPRLKKVSGVIDVANFGGLT
TQFMLSLDPARLVQYGVTLQQIKDAINANNGSAGGSLMNRGEQAYVVRGTGLIGSLQDMG
NVVVTAKSGTPVLVKDLGTLSYGNVERRGILGKDDNPDTVSGITLLLKDAQASQVIEGVH
AAVKDLNDNLLPKDVKVVPYLDRAALIGATTHTVGKTLAEGIVLVTLVLLLSLGSPRAAA
IVALTIPLSMLIAFVFMRHLNVPANLLSLGAIDFGILVDGAVVLVEGILRRREEAQDKPL
TPADAIAATVQVARPIFFGMLVIIAAYLPLFAFQRIEYKLFSPMAFAVGSALVGALLVAL
TLIPGLAYLAFRKPGRVFHNRPLEWLTLRYEAFLSRVLGRGRWVVATCGLALLGVIGLGG
TIGRDFLPYLDEGSLWLQVTMPPGITLEKATALANELRSATREFPEVSYIVTQTGRNDDG
TDPWTVSHIEASVGLHPYDTWKSGMSKQELIGKLAERYKQLPGFSVGFMQPMIDGVQDKL
SGAHSDLVVKIYGDSLDEMRRIAGDVIGVLEKVPGAADVVIDQEPPLPNLKIDVDRAAAA
RYGINVADVSDLISTGMGGAAIGQVYVGEKSYDMTVRFPEATRNDPDAIAGLMLTAPNGA
KVPLGQVASVRTVSGETTITRESSRRHLTVKLNARGRDLSSFLAQAQSAIERGVKYDHLR
YQIAWGGQFENLERAQARLMVILPLTLAIMFLLLFAEFGNLRQPAMVLLVVPLAMLGGLA
ALHLRGMTLNVSSAVGFIALFGVAVLNGVLMISQINRLRREDGRPLREAVIEGARSRIRP
VLVTATVAAFGLTPAMLAMGLGSDVQRPLATVVVGGLVTATLLTLVLLPALYHLVETRAE
AAARRRAEAETAAPDADLQEQHA