Protein Info for PS417_12535 in Pseudomonas simiae WCS417

Annotation: nitrilase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00795: CN_hydrolase
  • amino acids 5 to 277 (273 residues), 171 bits, E=1.6e-54

Best Hits

KEGG orthology group: K01501, nitrilase [EC: 3.5.5.1] (inferred from 94% identity to pfs:PFLU2708)

Predicted SEED Role

"Plant-induced nitrilase (EC 3.5.5.1), hydrolyses beta-cyano-L-alanine" (EC 3.5.5.1)

MetaCyc Pathways

• indole-3-acetate biosynthesis V (bacteria and fungi) (1/1 steps found)
• indole glucosinolate activation (herbivore attack) (8/13 steps found)
• indole-3-acetate biosynthesis II (5/12 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Cyanoamino acid metabolism
• Nitrogen metabolism
• Styrene degradation
• Tryptophan metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.5.1

Sequence Analysis Tools

See A0A1N7U391 at UniProt or InterPro

Protein Sequence (308 amino acids)

>PS417_12535 nitrilase (Pseudomonas simiae WCS417)
MPVSTVAALQIGSLPGGKGETLAQILSYEDEILRSGAQLVVMPEALLGGYPKGEAFGTQL
GYRLPEGREAFARYFDNAIDVPGPETQALAGLSARTGASLVLGVIERSGSTLYCTVLYFE
PAGGLVAKHRKLMPTGTERLIWGKGDGSTLPVIDTAVGRVGGAVCWENMMPLLRTAMYAK
GVEVWCAPTVDEREMWQVSMRHVAHEGRCFVVSACQVQASPEALGVQVANWPAERALIAG
GSVIVGPMGDILAGPLLGEAGLLTAQINTDDLVRARYDYDVVGHYARPDVFELVVDERAK
PGVRYITD