Protein Info for GFF2454 in Xanthobacter sp. DMC5

Annotation: Phenylalanine--tRNA ligase beta subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 804 TIGR00472: phenylalanine--tRNA ligase, beta subunit" amino acids 1 to 803 (803 residues), 727.9 bits, see alignment E=8.1e-223 PF01588: tRNA_bind" amino acids 45 to 146 (102 residues), 86.2 bits, see alignment E=3.3e-28 PF03483: B3_4" amino acids 217 to 388 (172 residues), 230 bits, see alignment E=3.6e-72 PF03484: B5" amino acids 407 to 471 (65 residues), 62.7 bits, see alignment E=7e-21 PF17759: tRNA_synthFbeta" amino acids 486 to 689 (204 residues), 151.5 bits, see alignment E=6.1e-48 PF03147: FDX-ACB" amino acids 710 to 803 (94 residues), 94.8 bits, see alignment E=9.1e-31

Best Hits

Swiss-Prot: 66% identical to SYFB_BRADU: Phenylalanine--tRNA ligase beta subunit (pheT) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: K01890, phenylalanyl-tRNA synthetase beta chain [EC: 6.1.1.20] (inferred from 89% identity to xau:Xaut_1930)

Predicted SEED Role

"Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20)" (EC 6.1.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.20

Use Curated BLAST to search for 6.1.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (804 amino acids)

>GFF2454 Phenylalanine--tRNA ligase beta subunit (Xanthobacter sp. DMC5)
MKFTYSWLKDHLEPTASFDQIVERLSLIGLEVEGVEDKAKQLAPFVVGEVLTAEKHPNAD
KLRVCTVSIGKGDPIQVVCGAPNARAGLKTVFAAPGTVIPATGTELKIGKIRDVESRGML
CSAREMGLSEEHDGILELPEDAPVGAPFAQVIGLDDPVIEIAVTPNRADCLGVSGVARDL
AAAGLGELMAPRVKPVDGTFPAPLKVTLEFGETSPLCPAFALRVVKGVKNGPSPQWLQDR
LRAIGLRPINALVDVTNFMTFDRNRPLHVFDLKKVTGNLVVRRAKGGETLLALDGKTYTL
DESMCVIADDKGVESLAGIMGGEDSGCDETTTDVVVESALWDAINIAQTGRKLGINSDAR
FRFERGVDPAFTVPGLDLATHLILELCGGEPSEMVLAGAVPDTARTIAFPLTEVKRLTGL
EASEDEIRGVLDRLGFAVSGAAPVIEVVPPSWRGDIEGKADLVEEVVRILGLERVPSTEL
PRGADARKAVLTTLQLRSRKARRALAARGMVEAVTWSFVSKDAARLFGGGADALALSNPI
ASDLSDMRPSLLPGLIKAAGANAARGAGDVALFEVGQVFLGDGPGDQRQAASGIRRGTAK
PSGGGRHWAGNAAAVDAFDAKADALAALAACGAPVANLQVTTDAPGWYHPGRSGTLRLGS
NSMAHFGEIHPAVLEALDVTGPLVAFEIVLDKIPEPKAKATRVKPNLDLSPFQAVKRDFA
FLVGRDVAAADILKAAQGADKKLVTGVGVFDLYEGKGIDPDKKSVAVEVTLQPRERTLTD
KEIEEVAGRIVAEVAKKTGATLRA