Protein Info for GFF2453 in Variovorax sp. SCN45

Annotation: NAD-dependent dihydropyrimidine dehydrogenase subunit PreT (EC 1.3.1.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF14691: Fer4_20
  • amino acids 30 to 137 (108 residues), 123.4 bits, E=2.2e-39
• PF03486: HI0933_like
  • amino acids 153 to 187 (35 residues), 24.8 bits, 5.1e-09
• PF07992: Pyr_redox_2
  • amino acids 153 to 442 (290 residues), 110.4 bits, E=6.3e-35
• PF00890: FAD_binding_2
  • amino acids 154 to 188 (35 residues), 22.6 bits, 3.2e-08
• PF13450: NAD_binding_8
  • amino acids 156 to 188 (33 residues), 36.4 bits, see alignment (E = 2.9e-12)
• PF00070: Pyr_redox
  • amino acids 285 to 351 (67 residues), 22.3 bits, E=8.6e-08

Best Hits

KEGG orthology group: K00266, glutamate synthase (NADPH/NADH) small chain [EC: 1.4.1.13 1.4.1.14] (inferred from 70% identity to aaa:Acav_0939)

Predicted SEED Role

"Pyridine nucleotide-disulphide oxidoreductase associated with reductive pyrimidine catabolism" in subsystem Pyrimidine utilization

MetaCyc Pathways

• L-glutamate and L-glutamine biosynthesis (7/7 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• superpathway of ammonia assimilation (plants) (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• ammonia assimilation cycle I (2/2 steps found)
• L-glutamate biosynthesis IV (1/1 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-glutamine degradation II (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.4.1.13, 1.4.1.14

Use Curated BLAST to search for 1.4.1.13 or 1.4.1.14

Protein Sequence (456 amino acids)

>GFF2453 NAD-dependent dihydropyrimidine dehydrogenase subunit PreT (EC 1.3.1.1) (Variovorax sp. SCN45)
MSSTMNPVAPTAHLASDVRAGRLDPAQYAKNFCDAHPPLNVVQANIEAERCYYCYDAPCQ
TACPTGIDIPTFIRRIADGNLRGAARTILEENPLGGMCARVCPTEVLCEQACVRNTNESK
PVEIGLLQRHATDAFFEKPGTPLFVRAADTGRRVAVVGAGPAGLACAHRLAVLGHQVVLF
DARPKLGGLNEYGLASYKTVNGFAQREIEWLLSVGGIEVRCNHALGRELHLDALAAEYDA
VFLGLGLAGVNALGLGEQTGVRDAVDFIAELRQAASPADVAIGRHVVVIGGGMTAVDAAV
QSRKLGAEDVSIVYRRGQESMGASADEQSWAKENGVRIIHWAAPKALRAKGGVLRGIDFA
RTALAGGKLVETGESLALQADMVLCAIGQSFIATVLEDSGVQLQGGRISTDTEGRSSRSK
VWAGGDARFGGRDLTVEAVEDGKQAALSIDRHLRAA