Protein Info for GFF2452 in Variovorax sp. SCN45

Annotation: NAD-dependent dihydropyrimidine dehydrogenase subunit PreA (EC 1.3.1.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 436 PF01180: DHO_dh" amino acids 4 to 306 (303 residues), 101.6 bits, see alignment E=1.9e-32 TIGR01037: dihydroorotate dehydrogenase family protein" amino acids 4 to 321 (318 residues), 169.6 bits, see alignment E=4.1e-54 PF01207: Dus" amino acids 123 to 180 (58 residues), 20.7 bits, see alignment E=8.2e-08 PF13237: Fer4_10" amino acids 339 to 392 (54 residues), 25.6 bits, see alignment 3.9e-09 PF14697: Fer4_21" amino acids 339 to 399 (61 residues), 72.4 bits, see alignment E=1.1e-23 PF12838: Fer4_7" amino acids 346 to 393 (48 residues), 34.7 bits, see alignment 8e-12

Best Hits

KEGG orthology group: K00207, dihydropyrimidine dehydrogenase (NADP+) [EC: 1.3.1.2] (inferred from 78% identity to aav:Aave_0967)

MetaCyc: 56% identical to (NADP)-dependent dihydropyrimidine dehydrogenase (Brevibacillus agri)
Dihydropyrimidine dehydrogenase (NADP(+)). [EC: 1.3.1.2]; 1.3.1.2 [EC: 1.3.1.2]

Predicted SEED Role

"Dihydropyrimidine dehydrogenase [NADP+] (EC 1.3.1.2)" in subsystem Pyrimidine utilization (EC 1.3.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (436 amino acids)

>GFF2452 NAD-dependent dihydropyrimidine dehydrogenase subunit PreA (EC 1.3.1.1) (Variovorax sp. SCN45)
MADLSIDFVGIRSPNPFWLASAPPTDKEINVVRAFEAGWGGVVWKTVGEDPNVVNVHGPR
YAVSYAADRRVTGFNNIELISDRPLRTNLEEMTRVKRNWPDRALVASVMVPCTEESWKNI
LPMIEDTGCDGLELNFGCPHGMSERGMGAAVGQVPEYIQMVAEWCKHYSKLPLIVKLTPN
VADIRPAARAAKKGGADAVSLINTINSIMGVDLDAMAMFPNTGGMGSHGGYCGPGAKPIA
LHMVAEIARDPQTAGLPISGIGGVSTWRDAAEFIALGSGSVQVCTAAMVYGFKIVQDLCD
GLSNFMDEKGYQNLEQLRGRAIGSITDWKELNLNHVDKAVIHQDACIQCGRCHVVCEDTS
HQAITATKDGMRYFEVKEEECVGCNLCVSICPVPGALSMRTLKPGEVDARTGHPVVAQYA
NWTTHPNNPMRADQHA