Protein Info for PS417_01250 in Pseudomonas simiae WCS417

Annotation: ATP-dependent DNA helicase RecQ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 645 transmembrane" amino acids 46 to 66 (21 residues), see Phobius details TIGR00614: ATP-dependent DNA helicase, RecQ family" amino acids 4 to 348 (345 residues), 372.6 bits, see alignment E=1.5e-115 PF00270: DEAD" amino acids 17 to 177 (161 residues), 76.1 bits, see alignment E=5.8e-25 PF04851: ResIII" amino acids 18 to 175 (158 residues), 25.9 bits, see alignment E=1.7e-09 PF00271: Helicase_C" amino acids 213 to 321 (109 residues), 77.1 bits, see alignment E=2.5e-25 PF16124: RecQ_Zn_bind" amino acids 512 to 555 (44 residues), 31.8 bits, see alignment 3.9e-11

Best Hits

KEGG orthology group: K03654, ATP-dependent DNA helicase RecQ [EC: 3.6.4.12] (inferred from 94% identity to pfs:PFLU0274)

Predicted SEED Role

"ATP-dependent DNA helicase, RecQ family" in subsystem DNA repair, bacterial RecFOR pathway

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7TU85 at UniProt or InterPro

Protein Sequence (645 amino acids)

>PS417_01250 ATP-dependent DNA helicase RecQ (Pseudomonas simiae WCS417)
MHDTLQQVFGFPQFRLGQEETVSAVLAGRSAAAIFPTGSGKSLCYQLSAVLLPHLTLVVS
PLLALMQDQLGFLQRHGISAGSIDSAQSRDEANDVMARARSGELKILMISVERLKNERFR
NFLQSVQISLLVVDEAHCISEWGHNFRPDYLKLPDYQRQFNIPQALLLTATATPKVIADM
QAKFAIAPGDVVTTGFYRPNLNLLVEPVSGADKRRRLVQWMSERAGQPSIVYVTLQKTAE
QIAEHLNRNGIQAEAYHAGLPHDKREGIQQRFMSGRSHCIVATIAFGMGIDKSDIRNVVH
FDLPKSIENYSQEIGRAGRDGQPSDCLVLANRDSLNVLENFVYGDTPEQEGIRRVLEELQ
AARADGQWEFLLRSLSDHSNIRELPLKTLLVQLELKGVIAPRYAFYAEYRFKYLVEPEAL
LARFSGERQQFVAAIIDVCKRAKIWATVDFDALYQQHNAERGRVVKALDYFQEQGLIELE
SKQMTEVYSLLDADFDADVLSKQLYTGFKQHEVGEVARIHAMLDLFATERCLGQRLAQYF
GDENAPQRCGHCSVCHGHVAHLAPPPGLPALVDKNFMGLCGDFIHRHHEHTGHLPGAERL
TRFLGGISVPLFTKLKARGIPGFAALEDYPYAEVREWAQAHLNDL