Protein Info for GFF2448 in Xanthobacter sp. DMC5

Annotation: Ribulose bisphosphate carboxylase large chain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02788: RuBisCO_large_N
  • amino acids 29 to 149 (121 residues), 147.7 bits, E=1.8e-47
• PF00016: RuBisCO_large
  • amino acids 159 to 466 (308 residues), 460.2 bits, E=3.2e-142

Best Hits

Swiss-Prot: 99% identical to RBL_XANFL: Ribulose bisphosphate carboxylase large chain (cbbL) from Xanthobacter flavus

KEGG orthology group: K01601, ribulose-bisphosphate carboxylase large chain [EC: 4.1.1.39] (inferred from 99% identity to xau:Xaut_1918)

MetaCyc: 61% identical to ribulose-bisphosphate carboxylase/oxygenase large subunit (Arabidopsis thaliana col)
Ribulose-bisphosphate carboxylase. [EC: 4.1.1.39]; 1.13.11.- [EC: 4.1.1.39]

Predicted SEED Role

"Ribulose bisphosphate carboxylase large chain (EC 4.1.1.39)" in subsystem CO2 uptake, carboxysome or Calvin-Benson cycle or Carboxysome or Photorespiration (oxidative C2 cycle) (EC 4.1.1.39)

MetaCyc Pathways

• Rubisco shunt (10/10 steps found)
• Calvin-Benson-Bassham cycle (12/13 steps found)
• ethene biosynthesis V (engineered) (20/25 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (21/27 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (20/26 steps found)
• oxygenic photosynthesis (13/17 steps found)
• photorespiration I (7/9 steps found)
• photorespiration III (7/9 steps found)
• photorespiration II (7/10 steps found)
• nucleoside and nucleotide degradation (archaea) (2/10 steps found)

KEGG Metabolic Maps

• Carbon fixation in photosynthetic organisms
• Glyoxylate and dicarboxylate metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.1.1.39

Use Curated BLAST to search for 4.1.1.39

Protein Sequence (488 amino acids)

>GFF2448 Ribulose bisphosphate carboxylase large chain (Xanthobacter sp. DMC5)
MGADAAIGQIKDAKKRYAAGVLKYAQMGYWDGDYQPKDTDILALFRVTPQDGVDPIEAAA
AVAGESSTATWTVVWTDRLTAADMYRAKAYKVEPVPGQPGQYFCWVAYELDLFEEGSIAN
LTASIIGNVFSFKPLKACRLEDMRLPVAYVKTFRGPPTGIVVERERLDKFGRPLLGATTK
PKLGLSGKNYGRVVYEGLKGGLDFVKDDENINSQPFMHWRDRFLYCMEAVNKAQAETGEV
KGHYLNITAGTMEEMYRRAEFAKELGSVVVMVDLIVGWTAIQSISNWCRENDVLLHMHRA
GHGTYTRQKGHGISFRVIAKWLRLAGVDHLHTGTAVGKLEGDPMTVQGYYNVCRETVTKT
DYTRGIFFDQDWAGLRKVMPVASGGIHAGQMHQLIDLFGEDVVLQFGGGTIGHPDGIQAG
AIANRVALETMILARNEGRDIKNEGPEILIEAAKWCQPLRAALDTWGEVTFNYASTDTSD
FVPTASVA