Protein Info for GFF2446 in Sphingobium sp. HT1-2

Annotation: Dihydrolipoamide dehydrogenase of 2-oxoglutarate dehydrogenase (EC 1.8.1.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01350: dihydrolipoyl dehydrogenase
  • amino acids 6 to 466 (461 residues), 545 bits, E=6.9e-168
• PF07992: Pyr_redox_2
  • amino acids 6 to 329 (324 residues), 267.4 bits, E=7.8e-83
• PF12831: FAD_oxidored
  • amino acids 7 to 51 (45 residues), 34.8 bits, 5.5e-12
• PF01134: GIDA
  • amino acids 7 to 144 (138 residues), 27 bits, E=1.1e-09
• PF00890: FAD_binding_2
  • amino acids 7 to 43 (37 residues), 26.7 bits, 1.5e-09
• PF00070: Pyr_redox
  • amino acids 176 to 251 (76 residues), 74.8 bits, E=2.8e-24
• PF02852: Pyr_redox_dim
  • amino acids 348 to 456 (109 residues), 129.6 bits, E=2.6e-41

Best Hits

Swiss-Prot: 59% identical to DLDH3_PSEPU: Dihydrolipoyl dehydrogenase 3 (lpd3) from Pseudomonas putida

KEGG orthology group: K00382, dihydrolipoamide dehydrogenase [EC: 1.8.1.4] (inferred from 95% identity to sch:Sphch_1350)

MetaCyc: 57% identical to glycine cleavage system L protein 1 (Arabidopsis thaliana col)
GCVMULTI-RXN [EC: 1.4.1.27]

Predicted SEED Role

"Dihydrolipoamide dehydrogenase of 2-oxoglutarate dehydrogenase (EC 1.8.1.4)" in subsystem TCA Cycle (EC 1.8.1.4)

MetaCyc Pathways

• folate transformations III (E. coli) (8/9 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (17/22 steps found)
• 2-oxoglutarate decarboxylation to succinyl-CoA (3/3 steps found)
• 2-oxoisovalerate decarboxylation to isobutanoyl-CoA (3/3 steps found)
• glycine biosynthesis II (3/3 steps found)
• glycine cleavage (3/3 steps found)
• glycine degradation (3/3 steps found)
• pyruvate decarboxylation to acetyl CoA I (3/3 steps found)
• folate transformations II (plants) (8/11 steps found)
• folate transformations I (8/13 steps found)
• photorespiration I (5/9 steps found)
• photorespiration III (5/9 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (5/9 steps found)
• photorespiration II (5/10 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glycine, serine and threonine metabolism
• Glycolysis / Gluconeogenesis
• Pyruvate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 1.8.1.4

Use Curated BLAST to search for 1.4.1.27 or 1.8.1.4

Protein Sequence (466 amino acids)

>GFF2446 Dihydrolipoamide dehydrogenase of 2-oxoglutarate dehydrogenase (EC 1.8.1.4) (Sphingobium sp. HT1-2)
MSDFDYDVLVIGAGPGGYVAAIRAAQLGLKTACAESRETLGGTCLNVGCIPSKALLHASE
LYDEAANGALAKLGVKIDKMSLDLPTMQGQRVDAIKGLTGGIEYLFKKNKVTWLKGLASF
TGANTVEVNGEKVTAKNIVIATGSSVAPLPGVAVDNAGGKIVDSTGALELDKVPGHLVVV
GGGVIGLELGSVWKRLGAKVTVVEYLDQILPGMDGEVRKEANKIFKKQGFEYKLGTKVTG
AEVGKSGVTLTVEPAAGGEAEKIEADVVLVSIGRRPNTEGLGLDKIGLELNARGQIETDH
EFGTKVPGVWAIGDVIPGPMLAHKAEDEGIAVAENIAGLTGIVNHDVIPSVVYTKPEIAG
VGLTEEAAKERGAVKVGKFPMMANSRAKTNHEPDGFVKIIADAETDKVLGVWIIATVAGT
MIAQAAQAMEFGASSEDIAYTCHAHPTHSEAIKEAAMAVTGKPIHM