Protein Info for GFF2442 in Sphingobium sp. HT1-2

Annotation: N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01128: IspD
  • amino acids 26 to 144 (119 residues), 31 bits, E=5.6e-11
• TIGR01173: UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase
  • amino acids 26 to 454 (429 residues), 487.3 bits, E=2.3e-150
• PF00483: NTP_transferase
  • amino acids 27 to 178 (152 residues), 41.2 bits, E=3.8e-14
• PF12804: NTP_transf_3
  • amino acids 27 to 150 (124 residues), 72.3 bits, E=1.4e-23
• PF00132: Hexapep
  • amino acids 285 to 319 (35 residues), 34 bits, 4.1e-12
  • amino acids 398 to 432 (35 residues), 35.2 bits, 1.7e-12
• PF14602: Hexapep_2
  • amino acids 399 to 432 (34 residues), 30.5 bits, 6e-11

Best Hits

Swiss-Prot: 69% identical to GLMU_SPHWW: Bifunctional protein GlmU (glmU) from Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273)

KEGG orthology group: K04042, bifunctional UDP-N-acetylglucosamine pyrophosphorylase / Glucosamine-1-phosphate N-acetyltransferase [EC: 2.3.1.157 2.7.7.23] (inferred from 86% identity to sch:Sphch_1346)

Predicted SEED Role

"N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)" in subsystem Peptidoglycan Biosynthesis or Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.3.1.157, EC 2.7.7.23)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (5/7 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• peptidoglycan recycling I (9/14 steps found)
• chitin biosynthesis (5/9 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (7/24 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (13/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.157 or 2.7.7.23

Protein Sequence (467 amino acids)

>GFF2442 N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157) (Sphingobium sp. HT1-2)
MAESRDIPDHLNEKDALAVTAPRPLAVVILAAGQGTRMKSGTHKVLHPIAGRPMLLHLLA
SVADLLPERQVVVVGAGREQVEKAVEGSGVAIAVQEQQLGTGHAVAQAHDALAGFAGDVL
ILYGDVPLVSGATMKAMLDRLSRGDEPRAVVLGFRPDDAAAYGRIIADGQGIIEKMVEFK
DASEAERAVTLCNSGLMAVRASDLFVLLDQIGNNNAAGEYYLPDIIMLPGANSAVIETEA
WEVAGVNSRAELAGVEAVWQDRRRAEAMREGVTLVAPQTVFFAYDTVLGRDVTVEPNVVF
GPGVTIADNVVIHAFSHLEGATVASGADIGPYARLRPGADIGPKAKVGNFVEIKKARLDE
GAKVNHLSYIGDASVGAAANIGAGTITCNYDGFFKYKTQIGAGAFIGSNSALVAPATIGD
GAIVAAGSVVTKPVPADALCLVRPQQESKPGWAAAFRTRMRERKAKA