Protein Info for GFF2434 in Xanthobacter sp. DMC5

Annotation: Glycine oxidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01266: DAO
  • amino acids 27 to 374 (348 residues), 251.1 bits, E=6.4e-78
• TIGR02352: glycine oxidase ThiO
  • amino acids 28 to 379 (352 residues), 351.1 bits, E=3.5e-109
• PF00890: FAD_binding_2
  • amino acids 28 to 282 (255 residues), 25.1 bits, E=2.6e-09
• PF13450: NAD_binding_8
  • amino acids 30 to 58 (29 residues), 25.5 bits, see alignment (E = 3.2e-09)

Best Hits

KEGG orthology group: K03153, glycine oxidase [EC: 1.4.3.19] (inferred from 82% identity to xau:Xaut_2223)

Predicted SEED Role

"Glycine oxidase ThiO (EC 1.4.3.19)" in subsystem Thiamin biosynthesis (EC 1.4.3.19)

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis II (9/11 steps found)
• glyphosate degradation II (1/1 steps found)
• thiazole component of thiamine diphosphate biosynthesis II (5/7 steps found)
• butanol and isobutanol biosynthesis (engineered) (3/8 steps found)

KEGG Metabolic Maps

• Thiamine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.3.19

Protein Sequence (401 amino acids)

>GFF2434 Glycine oxidase (Xanthobacter sp. DMC5)
MSESLSQFTSAPSSQAGSAPAGKAKPRVAIVGGGVIGLALAWRLADAGCPVDLFEAGETG
EGASRAAAGMLAACAEAEPGEEGLLALNCASQTLWPAFAAELERASGEVVDLRTEGTITI
ALTADDLAKLRHLYAFQQGLGLPVQWLTAAEVRRREPYLAPRLAGGVLSPEDHQVDNRKV
AAALKIAALKAGATIHQNTPVSRVETAGGRATGIVTGETHHAADVVVLAAGAWSRGVDIT
PARPLPVRPIKGQMLSLRMDPAAPILSHVLWAPGSYLVPRRDGRLIIGATTEEKGFDTDL
TAGGQLALLTNAWRALPTLEELSIVEQWVGFRPGSRDDAPILGPSPEVDGLVYATGHHRN
GILLLPVTTQVIADHILGGKIADVALPFGAERFGPPRAAAE