Protein Info for GFF2414 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: Ribosomal RNA large subunit methyltransferase N (EC 2.1.1.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00048: 23S rRNA (adenine(2503)-C(2))-methyltransferase
  • amino acids 18 to 373 (356 residues), 586.8 bits, E=6.5e-181
• PF21016: RlmN_N
  • amino acids 22 to 81 (60 residues), 109.2 bits, E=6.7e-36
• PF04055: Radical_SAM
  • amino acids 124 to 296 (173 residues), 61.9 bits, E=8.9e-21

Best Hits

Swiss-Prot: 100% identical to RLMN_SALTY: Dual-specificity RNA methyltransferase RlmN (rlmN) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K06941, ribosomal RNA large subunit methyltransferase N [EC: 2.1.1.-] (inferred from 100% identity to sek:SSPA0322)

MetaCyc: 96% identical to 23S rRNA m2A2503 methyltransferase/tRNA m2A37 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN-11586 [EC: 2.1.1.192]; 2.1.1.- [EC: 2.1.1.192]

Predicted SEED Role

"Ribosomal RNA large subunit methyltransferase N (EC 2.1.1.-)" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase (EC 2.1.1.-)

KEGG Metabolic Maps

• Alkaloid biosynthesis I
• Anthocyanin biosynthesis
• Benzoxazinone biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway
• Carotenoid biosynthesis - General
• Flavonoid biosynthesis
• Histidine metabolism
• Insect hormone biosynthesis
• Naphthalene and anthracene degradation
• Phenylpropanoid biosynthesis
• Porphyrin and chlorophyll metabolism
• Tryptophan metabolism
• Tyrosine metabolism
• Ubiquinone and menaquinone biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.- or 2.1.1.192

Protein Sequence (388 amino acids)

>GFF2414 Ribosomal RNA large subunit methyltransferase N (EC 2.1.1.-) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MSEQIVTPESSTPVVPNKETKINLLDLNRQQMREFFKNLGEKPFRADQVMKWMYHYCCDN
FDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQRVETVYIPEDDRAT
LCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVGAAKVTGQRPITNVVMM
GMGEPLLNLTNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDMIDVALAISLHA
PNDTIRDEIVPINKKYNIETFLGAVRRYLEKSNANQGRVTIEYVMLDHVNDGTEHAHQLA
ELLKETPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVLMSYGFTTIVRKTRGDDIDAACG
QLAGDVIDRTKRTLRKRMQGEVIDIKAI