Protein Info for PS417_12270 in Pseudomonas simiae WCS417

Annotation: pyruvate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02776: TPP_enzyme_N
  • amino acids 2 to 112 (111 residues), 105.3 bits, E=2.7e-34
• PF00205: TPP_enzyme_M
  • amino acids 186 to 314 (129 residues), 113.2 bits, E=1.2e-36
• PF02775: TPP_enzyme_C
  • amino acids 376 to 522 (147 residues), 148.5 bits, E=2e-47

Best Hits

Swiss-Prot: 66% identical to POXB_ECOLI: Pyruvate dehydrogenase [ubiquinone] (poxB) from Escherichia coli (strain K12)

KEGG orthology group: K00156, pyruvate dehydrogenase (quinone) [EC: 1.2.5.1] (inferred from 98% identity to pfs:PFLU2653)

MetaCyc: 66% identical to pyruvate oxidase (Escherichia coli K-12 substr. MG1655)
RXN-11496 [EC: 1.2.5.1]; RXN0-2022 [EC: 1.2.5.1]

Predicted SEED Role

"Pyruvate oxidase [ubiquinone, cytochrome] (EC 1.2.2.2)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 1.2.2.2)

MetaCyc Pathways

• pyruvate to cytochrome bo oxidase electron transfer (2/2 steps found)
• pyruvate to cytochrome bd oxidase electron transfer (1/2 steps found)
• pyruvate fermentation to acetoin (1/3 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.2.2 or 1.2.5.1

Sequence Analysis Tools

See A0A1N7UB93 at UniProt or InterPro

Protein Sequence (569 amino acids)

>PS417_12270 pyruvate dehydrogenase (Pseudomonas simiae WCS417)
MAQQLATTLEQAGIKRIWGLTGDSLNGLTDALRTMDSIEWMHVRHEEVAAFAAGAEAAAT
GELTVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAQIPSSEIGLNYFQETHPQELFKEC
SHFIELVTNPEQMPHVLHRAMRSAILNRGVAVVVIPGDVSLLEVEDKLKPWPALHAPRTL
PADQDLQRLIEILESSQKTTLLCGSGCAGAHDQVVALADALGAPVVHALRGKEHVEWDNP
FDVGMTGLIGFSSGYHAMLDCDTLIMLGTDFPYRQFYPTDAKIIQIDRNPQALGRRATLD
LGIAADVSETIGALLPRLTRKTDRSFLETSLKHYEKARQGLDDLAQPSKANRPIHPQYVA
RLLSELADDDAIFTADVGSPTVWAARYLKMNGKRRLIGSFNHGSMANAMPQAIGAQAAFP
DRQVISMSGDGGFAMLMGDFISLAQLNLPVKVIVFDNSSLGFVAMEMKAAGYLDAGTELK
NPDFAAMSNAMGILGIRVEQSEDLEPALRRALAHDGPVLVDVVTATQELVMPPTIKLEQA
KGFSLYMLKAVMSGRGDEVIELARTNWLR