Protein Info for GFF2406 in Sphingobium sp. HT1-2

Annotation: Xylan 1,4-beta-xylosidase (EC 3.2.1.37)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 556 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF04616: Glyco_hydro_43" amino acids 46 to 336 (291 residues), 284.8 bits, see alignment E=7.8e-89 PF17851: GH43_C2" amino acids 371 to 548 (178 residues), 117 bits, see alignment E=9.4e-38

Best Hits

KEGG orthology group: None (inferred from 66% identity to aex:Astex_0642)

Predicted SEED Role

"Beta-xylosidase (EC 3.2.1.37)" in subsystem Xylose utilization (EC 3.2.1.37)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.37

Use Curated BLAST to search for 3.2.1.37

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (556 amino acids)

>GFF2406 Xylan 1,4-beta-xylosidase (EC 3.2.1.37) (Sphingobium sp. HT1-2)
MRKTRRAALLLLAAIAGPALAGPARFSDFRYDGRAQEQVTPGPDDYRNPILSGYYPDPSI
TRVGEAYYLVNSSFAHYPGLPVFRSSDMVHWRQIGNAIDRPDQFDFSGLRSSRGMFAPDI
SFHDGLFYLVGTCVDCGGNFVMTAKDAAGPWSKPHWLPFEGIDPSIYWEGERAFIVNNRA
PAEPPRYDGHRAIWVQEFDWHALRMTGTATQIVNGGVDISQKPSWIEGPHLLKREGFYYL
IAAEGGTGDQHSEVVFRARDLRGPFTPYAGNPILSQRSLDPARPHPVTSAGHAKLVQTQA
GDWWATFLATRPYGPDLYNIGRETFLLPVEWRDGWPHILDAGKAIPYAAKRPPLPVRDTD
APPTSGDFAYRDRFDGPRLDMGWIGLRTPKAPLYRLETGELVLDGATALGDLSGAPAFVG
RRQQHHIATISTDLTYRPETEGDRAGLAAVQSGRSFLFFGVTQKDGKPQIALMLREDSDG
DRLVAAAPIDPAKPVRLTIALDGGTARFAYGFDGRETVLARDVDIRFLSTHEAGGFVGTV
VGPYAWSARVTRPISN