Protein Info for GFF2404 in Variovorax sp. SCN45

Annotation: Hypothetical protein with Rieske (2Fe-2S) region

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00355: Rieske
  • amino acids 42 to 122 (81 residues), 69.3 bits, E=2.2e-23
• PF19112: VanA_C
  • amino acids 183 to 352 (170 residues), 77.3 bits, E=1.9e-25

Best Hits

KEGG orthology group: K03862, vanillate monooxygenase [EC: 1.14.13.82] (inferred from 77% identity to bxe:Bxe_C1389)

Predicted SEED Role

"Vanillate O-demethylase oxygenase subunit (EC 1.14.13.82)" in subsystem Phenylpropanoid compound degradation (EC 1.14.13.82)

MetaCyc Pathways

• vanillin and vanillate degradation II (2/2 steps found)

KEGG Metabolic Maps

• 2,4-Dichlorobenzoate degradation

Isozymes

Compare fitness of predicted isozymes for: 1.14.13.82

Use Curated BLAST to search for 1.14.13.82

Protein Sequence (360 amino acids)

>GFF2404 Hypothetical protein with Rieske (2Fe-2S) region (Variovorax sp. SCN45)
MTNVNASQTIPIQAMHSNVLPEGFLPRGCTFAAQDWPILAKHWFPVARAVDVQDQPLQAT
LLDLELVVYRTPGGIHVGRDLCPHRGVPLSMGCVEGEELVCAYHGLRFGPDGQCRKIPAQ
PELVPPARFRVGMFPAVERYGLVWTCLLPGADTAIPPMPSWTDPAYQPILPPPVDIAGSA
GRQVEGFVDVAHFAFVHHEAFADRDNPVVPAYNTEVTDFGLRSEYWSTVSNYPKSLQHLA
PPGFQWLRSFEVHPPFTAQLTVHFPEEGRLHILNAASPVSARKTRLFVPIARNFDRNGSL
EDVYAFNAQVFAEDQAIIERQRPEDLPLDMQEEAHFAADRTSAGYRRLLKGMGLGLASTR