Protein Info for GFF2400 in Xanthobacter sp. DMC5

Annotation: 2-isopropylmalate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 520 TIGR00973: 2-isopropylmalate synthase" amino acids 12 to 504 (493 residues), 698.5 bits, see alignment E=2.1e-214 PF00682: HMGL-like" amino acids 12 to 281 (270 residues), 316.7 bits, see alignment E=1.9e-98 PF22617: HCS_D2" amino acids 295 to 376 (82 residues), 102.8 bits, see alignment E=1.6e-33 PF08502: LeuA_dimer" amino acids 378 to 506 (129 residues), 135.7 bits, see alignment E=1.4e-43

Best Hits

Swiss-Prot: 77% identical to LEU1_RHOP5: 2-isopropylmalate synthase (leuA) from Rhodopseudomonas palustris (strain BisA53)

KEGG orthology group: K01649, 2-isopropylmalate synthase [EC: 2.3.3.13] (inferred from 93% identity to xau:Xaut_1329)

Predicted SEED Role

"2-isopropylmalate synthase (EC 2.3.3.13)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 2.3.3.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.3.13

Use Curated BLAST to search for 2.3.3.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (520 amino acids)

>GFF2400 2-isopropylmalate synthase (Xanthobacter sp. DMC5)
MTASNTVGANDRVIIFDTTLRDGEQCPGASMTFEEKLEVAEVLDEMGVDVIEAGFPIASI
GDFESVSEIARRTKNATIAGLSRAALNDIDRCAEAVKHARRGRIHTFLSTSPVHMKYKLQ
KEPHEVLEMVVASVTRARNHVEDVEWSSEDGTRTEIDFLCRCVEAAIKAGATTINIPDTV
GYTTPGEYEALFRTVRERVPNSDKAIFSVHCHNDLGMAVANSLAGLAGGARQIECTINGI
GERAGNAALEEIVMAIDTRSDVLPYRTGINSKMLTRVSKLVSAVTSFPVQYNKAIVGRNA
FAHESGIHQDGMLKHTQTYEIMTPESVGVSKTSLVMGKHSGRAAFRDKLKAMGYELGENA
LNDAFTRFKDLADRKKVVYDEDIEALVDQEIAVSHDRIKLLSLSVIAGTHGPQRATMRVE
VDGRVITEEAEGNGPVDATFNAIKALVPHQAKLDLYQVHAVTEGTDAQAEVSVRLEENGK
VVTARAANPDTLVASAQAYITALNKLQMKRQSMNAQSAAS