Protein Info for GFF240 in Sphingobium sp. HT1-2

Annotation: ABC transporter, substrate-binding protein (cluster 5, nickel/peptides/opines)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 502 signal peptide" amino acids 1 to 32 (32 residues), see Phobius details PF00496: SBP_bac_5" amino acids 81 to 175 (95 residues), 35.9 bits, see alignment E=2.5e-13

Best Hits

KEGG orthology group: K02035, peptide/nickel transport system substrate-binding protein (inferred from 71% identity to sch:Sphch_2873)

Predicted SEED Role

"Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein OppA (TC 3.A.1.5.1)" in subsystem ABC transporter oligopeptide (TC 3.A.1.5.1) or Sex pheromones in Enterococcus faecalis and other Firmicutes (TC 3.A.1.5.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (502 amino acids)

>GFF240 ABC transporter, substrate-binding protein (cluster 5, nickel/peptides/opines) (Sphingobium sp. HT1-2)
MFAVPPRLRRLSRRAALIAGASLGLALGSCSDEGSGPVVVSVIGNRTELAKPLQNLPNPA
AKLILEATAQGLVAFDAGGDILPALAQRWVVEDDGRSYIFRLRRAFWANGARVTAPDVAR
MLMARIEMLRQLDPDGPLDAVQAVVPMTGEVIEIRMAAARPYVLQMLAQPQMAVLSRDGG
TGPYRSVWRSGAAMLAPVDRLSGDDADSDEEEAGVPAWQTRVLRAERASLAIIRFREKQS
ALVLGGRYADLPLLVPAGIDRDAVRMDPVQGLLGLAVTGKGDLLDDDGVRAAINMAVDRN
QLLQMFPLGGWAISEQLLPGQLDLPVAPPAPAWVGQAMDDRRAQGRAIIDRWRGTHGDPP
PLRIALPAGPGSTLLFGLLARDLGAIGLAAERVDLADKADLSLIDEVAAYDSALWYLGRI
GCARKIHCSPEGEAALQAASLASTAEERAARVAQAVGLIQGHDGYIPLGAPIRWSLVSKR
LSGFLPSPRGRHPLNHLFRSTN