Protein Info for GFF2398 in Sphingobium sp. HT1-2

Annotation: Peptidyl-prolyl cis-trans isomerase (EC 5.2.1.8)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 259 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF00160: Pro_isomerase" amino acids 77 to 213 (137 residues), 140.8 bits, see alignment E=2.4e-45

Best Hits

KEGG orthology group: K01802, peptidylprolyl isomerase [EC: 5.2.1.8] (inferred from 82% identity to sjp:SJA_C1-33410)

Predicted SEED Role

"Peptidyl-prolyl cis-trans isomerase (EC 5.2.1.8)" in subsystem Queuosine-Archaeosine Biosynthesis (EC 5.2.1.8)

Isozymes

Compare fitness of predicted isozymes for: 5.2.1.8

Use Curated BLAST to search for 5.2.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (259 amino acids)

>GFF2398 Peptidyl-prolyl cis-trans isomerase (EC 5.2.1.8) (Sphingobium sp. HT1-2)
MRFTSALKTVAIGFALTASSVAFAQGGGGGGGGAEEMKKAETAAKAEQNAKSLGAQLTPQ
LPPATIPADPQNIWDLDLSSGGRVRIQLRPDIAPNHVERIKELTRQGFYNGLKFHRVIPG
FMAQGGDPKGDGTGGSTLPDLKAEFNPMPHLRGTLSMARAQSEDSANSQFFIVLLPRMQL
DKKYTVFGRVIEGMQYVDAIHEGEPPADPTVILQASIESDGKPPVLAPPPPPPPPAPEPA
ALPSKKPAAPAKKPAPKKK