Protein Info for GFF2397 in Sphingobium sp. HT1-2

Annotation: S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 2.4.99.17)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 342 TIGR00113: S-adenosylmethionine:tRNA ribosyltransferase-isomerase" amino acids 1 to 340 (340 residues), 361 bits, see alignment E=2.8e-112 PF02547: Queuosine_synth" amino acids 4 to 340 (337 residues), 404.6 bits, see alignment E=1.5e-125

Best Hits

Swiss-Prot: 85% identical to QUEA_SPHWW: S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) from Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273)

KEGG orthology group: K07568, S-adenosylmethionine:tRNA ribosyltransferase-isomerase [EC: 5.-.-.-] (inferred from 92% identity to sch:Sphch_1305)

Predicted SEED Role

"S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 5.-.-.-)" in subsystem Queuosine-Archaeosine Biosynthesis (EC 5.-.-.-)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (342 amino acids)

>GFF2397 S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 2.4.99.17) (Sphingobium sp. HT1-2)
MRVDLFDFDLPAENIALRPASPRDSARLLLVPGDGAMEDRIVRDLPSLLRAGDVLVFNDT
RVIPAQLEGMRGEARIGATLHKRLGLRQWQAFLRNAKRVRDGDRIDFGAGVTAIAGPRDE
DGGVTLDFEGEEPVEILLERAGRMPLPPYIASKRATDERDRSDYQTMFAREDGAVAAPTA
ALHFTPDLTAALAQAGVLTETLTLHVGAGTFLPVKADDTDDHRMHAEWGRIDQDTADRLN
AARAAGGRIIAVGTTSLRLLESATGEDGVIRPFADETRIFITPGYRFRAIDGLMTNFHLP
KSTLFMLVSALMGRDRMQQVYAHAIAAGYRFYSYGDSSLLLP