Protein Info for GFF2389 in Xanthobacter sp. DMC5

Annotation: Sulfite reductase [ferredoxin]

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03460: NIR_SIR_ferr
  • amino acids 52 to 111 (60 residues), 72.5 bits, 1.9e-24
  • amino acids 343 to 396 (54 residues), 53.7 bits, 1.4e-18
• PF01077: NIR_SIR
  • amino acids 119 to 271 (153 residues), 155.2 bits, E=1e-49
  • amino acids 410 to 545 (136 residues), 49.3 bits, E=4e-17

Best Hits

KEGG orthology group: K00381, sulfite reductase (NADPH) hemoprotein beta-component [EC: 1.8.1.2] (inferred from 91% identity to xau:Xaut_1397)

Predicted SEED Role

"Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2)" in subsystem Cysteine Biosynthesis (EC 1.8.1.2)

MetaCyc Pathways

• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
• assimilatory sulfate reduction I (4/4 steps found)
• assimilatory sulfate reduction III (3/3 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (7/10 steps found)

KEGG Metabolic Maps

• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.8.1.2

Use Curated BLAST to search for 1.8.1.2

Protein Sequence (550 amino acids)

>GFF2389 Sulfite reductase [ferredoxin] (Xanthobacter sp. DMC5)
MYVYDEFDRAFLQERVVEFRDQVERRLAGELNEEEFKPLRLMNGVYLQLHAYMLRVCIPY
GTLSARQLRALAAVARKYDRGYGHFTTRQNIQFNWIKLAELPDAMADLAEVGIHGIQTSG
NCIRNTTTDPWAGAAADEVEDPRLYAEVIRQWSTLHPEFTYLPRKFKIAITAAKHDRAAI
RVHDVGLRLHKRDGKTGFEVMVGGGLGRSPYIGKTIRDFLPEEDLLSYLEAILRTYNLGG
RRDNIYKARIKIQVHEMGAEAFAQKVEAEWAEIRDGALKLDKAALDAIRDRFLNPALEDL
EDAPAVLATALADPDFARWHANSVAPHKAKGHAIVTLSLKPVGAPPGDATADQMDAIADL
ADEYAYGEIRVAHEQNLTLPYVRQKDLPALWAKLKAIGVATPNVGLISDIIACPGLDYCS
LANARSIPVAQKLTERFADDARIADIGRLHLNISGCINACGHHHVGHIGILGVEKKDEEF
FQITLGGKGNSKARIGELIGPAVSEGEVVDVVERLVETYLDLRTSPAETFIAAVQRLGTD
PFKERAYAPR