Protein Info for GFF2383 in Xanthobacter sp. DMC5

Annotation: Sulfate/thiosulfate import ATP-binding protein CysA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 347 TIGR00968: sulfate ABC transporter, ATP-binding protein" amino acids 3 to 240 (238 residues), 384.3 bits, see alignment E=1.1e-119 PF00005: ABC_tran" amino acids 18 to 164 (147 residues), 132.8 bits, see alignment E=2.7e-42 PF08402: TOBE_2" amino acids 276 to 341 (66 residues), 24 bits, see alignment E=7e-09 PF12857: TOBE_3" amino acids 285 to 340 (56 residues), 36.8 bits, see alignment E=5.9e-13

Best Hits

Swiss-Prot: 64% identical to CYSA_BRADU: Sulfate/thiosulfate import ATP-binding protein CysA (cysA) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: K02045, sulfate transport system ATP-binding protein [EC: 3.6.3.25] (inferred from 91% identity to xau:Xaut_1391)

Predicted SEED Role

"Sulfate and thiosulfate import ATP-binding protein CysA (EC 3.6.3.25)" in subsystem Cysteine Biosynthesis (EC 3.6.3.25)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.3.25

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (347 amino acids)

>GFF2383 Sulfate/thiosulfate import ATP-binding protein CysA (Xanthobacter sp. DMC5)
MTIDVVGVTKRFKTFAALDNVSLTIRTGELVALLGPSGSGKTTLLRIIAGLEWPDSGEVR
FDGEDALSRSVRERNVGFVFQHYALFRHMNVFENVAFGLRVRRGADKLPEAAIRAKVNEL
LHLVQIDWLAERYPAQLSGGQRQRVALARALAISPKVLLLDEPFGALDAKVRKELRRWLR
HLHEELPVTSVLVTHDQEEALELADRVVVMGHGKIEQVGSPGEVYDNPATAFVHDFIGET
IALPGEVKGGALYLGGRPLGLDPQGVADGPARILARPHDVDLADPSSTAISGKVLSVRTF
GPTRRADVELAGSSGPVVVEAAVPHGQDLAPGTQVGLKPRRYKVFAA