Protein Info for GFF2376 in Xanthobacter sp. DMC5

Annotation: Formate--tetrahydrofolate ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01268: FTHFS
  • amino acids 13 to 565 (553 residues), 849.1 bits, E=6.2e-260

Best Hits

Swiss-Prot: 65% identical to FTHS_PARDP: Formate--tetrahydrofolate ligase (fhs1) from Paracoccus denitrificans (strain Pd 1222)

KEGG orthology group: K01938, formate--tetrahydrofolate ligase [EC: 6.3.4.3] (inferred from 85% identity to xau:Xaut_1383)

MetaCyc: 58% identical to formyltetrahydrofolate synthetase subunit (Moorella thermoacetica)
Formate--tetrahydrofolate ligase. [EC: 6.3.4.3]

Predicted SEED Role

"Formate--tetrahydrofolate ligase (EC 6.3.4.3)" in subsystem Folate Biosynthesis or One-carbon metabolism by tetrahydropterines or Serine-glyoxylate cycle (EC 6.3.4.3)

MetaCyc Pathways

• folate transformations III (E. coli) (9/9 steps found)
• folate transformations II (plants) (10/11 steps found)
• folate polyglutamylation (5/5 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
• formaldehyde oxidation VII (THF pathway) (4/4 steps found)
• formate assimilation into 5,10-methylenetetrahydrofolate (3/3 steps found)
• folate transformations I (10/13 steps found)
• tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate (2/2 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (6/9 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• reductive acetyl coenzyme A pathway I (homoacetogenic bacteria) (4/10 steps found)
• L-histidine degradation III (1/6 steps found)
• purine nucleobases degradation I (anaerobic) (6/15 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (25/56 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• One carbon pool by folate

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.4.3

Protein Sequence (565 amino acids)

>GFF2376 Formate--tetrahydrofolate ligase (Xanthobacter sp. DMC5)
MHDRSARPLDPTSDAAIAASVTPRPIEDVAADLGIPADALYRYGPHKGKISLDFVAEAES
RPRGQLVLVTAINPTPAGEGKTTTTIGLGDALSQLGTSTAVCLREPSLGPVFGAKGGATG
GGRAQIIPMQDINLHFTGDLHAITAAHNLLSALIDNHIHWGNALDLDARRISWRRVMDLN
DRALRQITVGLGGPANGYPREDGFDITVASEVMAILCLADSLSDLEARLSRMVIGQTRGR
RMVRAEELGGIGAMVALLKDAFQPNLVQTLEGTPALVHGGPFANIAHGCNSVMATRTALG
LADVVVTEAGFGADLGAEKFLDIKCRQAGLVPSAAVVVATVRALKMHGGVPLKELGTPDA
AAVTLGCGNLKRHVENLKKFGLPVVVALNHFTGDTPEELEAVRAALVAEGVDVFPCGHWA
RGGEGALDLARAVLDLLARRPKFEYLYPSELPLREKIETIAREIYRAERVDFSSAATAKL
KEYELGGFGDLPVCMAKTQYSFSADPTARNAPTGYTLPIREVRLSAGAGFVVALAGDIMT
MPGLPSAPAAQDIFVMPDGFIAGLS