Protein Info for GFF2374 in Xanthobacter sp. DMC5

Annotation: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00288: GHMP_kinases_N
  • amino acids 68 to 143 (76 residues), 43.4 bits, E=3.2e-15
• PF08544: GHMP_kinases_C
  • amino acids 212 to 275 (64 residues), 34.1 bits, E=3e-12

Best Hits

Swiss-Prot: 75% identical to ISPE_XANP2: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) from Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2)

KEGG orthology group: K00919, 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase [EC: 2.7.1.148] (inferred from 75% identity to xau:Xaut_1381)

Predicted SEED Role

"4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (EC 2.7.1.148)" in subsystem Isoprenoid Biosynthesis or polyprenyl synthesis (EC 2.7.1.148)

MetaCyc Pathways

• methylerythritol phosphate pathway II (9/9 steps found)
• superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP) (11/12 steps found)
• taxadiene biosynthesis (engineered) (11/13 steps found)
• methylerythritol phosphate pathway I (8/9 steps found)
• isoprene biosynthesis I (8/10 steps found)
• superpathway of ergosterol biosynthesis II (10/26 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Terpenoid biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.148

Protein Sequence (289 amino acids)

>GFF2374 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (Xanthobacter sp. DMC5)
METLRTRAPAKINLTLRIIGRRTDGFHDLASLVVFSGAADLLSLTPDAALSLTVKGRTAA
AAGPDADNLVLRAARAFSAEVPGAQLGAFLLEKRLPVAAGLGGGSSDAAAALRLLGRLNG
LAPDDPRLFAAARATGSDVPVCLDPRARLMEGVGDVLSPPLALPPLAGVLVNCGVAVPTA
AVFRQLGLAPGGAFDGPSLPAVLPADRAELVAAIAAVPNDLEPPAVAVAPKIAEVIDRLA
AQPEARLVRMSGSGATVFALTDDCRAAAALARRVLAAEPDWWVKPTILR