Protein Info for GFF2369 in Xanthobacter sp. DMC5

Annotation: Glutamine-dependent NAD(+) synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00795: CN_hydrolase
  • amino acids 9 to 225 (217 residues), 103.1 bits, E=2.6e-33
• PF02540: NAD_synthase
  • amino acids 274 to 528 (255 residues), 240.2 bits, E=2.7e-75
• TIGR00552: NAD+ synthetase
  • amino acids 278 to 530 (253 residues), 200 bits, E=1.9e-63

Best Hits

KEGG orthology group: K01916, NAD+ synthase [EC: 6.3.1.5] (inferred from 82% identity to xau:Xaut_1377)

Predicted SEED Role

"NAD synthetase (EC 6.3.1.5) / Glutamine amidotransferase chain of NAD synthetase" in subsystem NAD and NADP cofactor biosynthesis global or NAD regulation (EC 6.3.1.5)

MetaCyc Pathways

• aspartate superpathway (22/25 steps found)
• NAD de novo biosynthesis I (6/6 steps found)
• NAD salvage pathway II (PNC IV cycle) (5/5 steps found)
• NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde (4/4 steps found)
• NAD salvage pathway I (PNC VI cycle) (6/7 steps found)
• NAD de novo biosynthesis III (5/6 steps found)
• NAD de novo biosynthesis IV (anaerobic) (5/6 steps found)
• NAD salvage pathway V (PNC V cycle) (4/5 steps found)
• NAD salvage (plants) (6/11 steps found)
• NAD de novo biosynthesis II (from tryptophan) (4/9 steps found)
• superpathway of NAD biosynthesis in eukaryotes (6/14 steps found)

KEGG Metabolic Maps

• Nicotinate and nicotinamide metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.1.5

Use Curated BLAST to search for 6.3.1.5

Protein Sequence (554 amino acids)

>GFF2369 Glutamine-dependent NAD(+) synthetase (Xanthobacter sp. DMC5)
MTEPADTLLIAIAQLNPTVGDVSGNLAIARRTRQTAGEAGADLVVFPELFIAGYPAEDLV
LKPAFLAACQEAVEALAAETATGPAVLIGAPWVEEGRLFNSVLLLEEGKISAVRHKVDLP
NYGVFDEKRVFAAGPLPGPIPFKGVRLGVPICEDIWGPDVVECLAETGAEILIVPNGSPY
RRSVHAERENIAVARVVESHLPLLYVNQVGGQDELVFDGASFVLNADRFPALQFPGFVER
VRLSRWQRFEDTWRCLGGNLVEAVEGDEADYAACVVGLRDYVDKNGFPGVVLGLSGGIDS
ALCAAMAVDALGRERVRCLMLPYRFTSEDSLKDAAAVAEALGVSYEVVPIAPAVEGLEAV
LAPLFAGTERDVTEENLQARVRGTLLMSVSNKFGAMVVTTGNKSEMSTGYATLYGDMNGG
FNPIKDLYKTEVLRLAHLRNRWKPAEALGPDGIVIPEPLIAKPPSAELRADQKDEDALPP
YPLLDTILARLVEGESALDEIIADGFDAATVARVARLLAGAEYKRRQAAPGVKVTGRSFG
RDRRYPITNRFREA