Protein Info for GFF2368 in Sphingobium sp. HT1-2

Annotation: Triosephosphate isomerase (EC 5.3.1.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00121: TIM
  • amino acids 5 to 245 (241 residues), 256.8 bits, E=1.1e-80
• TIGR00419: triose-phosphate isomerase
  • amino acids 6 to 236 (231 residues), 156.6 bits, E=4.5e-50

Best Hits

Swiss-Prot: 53% identical to TPIS_RUEPO: Triosephosphate isomerase (tpiA) from Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3)

KEGG orthology group: K01803, triosephosphate isomerase (TIM) [EC: 5.3.1.1] (inferred from 77% identity to sjp:SJA_C1-16120)

Predicted SEED Role

"Triosephosphate isomerase (EC 5.3.1.1)" in subsystem Calvin-Benson cycle or Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes or MLST (EC 5.3.1.1)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
• homolactic fermentation (11/12 steps found)
• glycolysis III (from glucose) (10/11 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (14/17 steps found)
• gluconeogenesis I (11/13 steps found)
• formaldehyde assimilation III (dihydroxyacetone cycle) (10/12 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (15/19 steps found)
• superpathway of anaerobic sucrose degradation (15/19 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (17/22 steps found)
• glycolysis II (from fructose 6-phosphate) (9/11 steps found)
• glycolysis I (from glucose 6-phosphate) (10/13 steps found)
• gluconeogenesis III (9/12 steps found)
• glycolysis VI (from fructose) (8/11 steps found)
• glycolysis IV (7/10 steps found)
• Calvin-Benson-Bassham cycle (9/13 steps found)
• superpathway of N-acetylneuraminate degradation (15/22 steps found)
• superpathway of hexitol degradation (bacteria) (12/18 steps found)
• ethene biosynthesis V (engineered) (17/25 steps found)
• glycolysis V (Pyrococcus) (6/10 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (18/27 steps found)
• glycerol degradation to butanol (10/16 steps found)
• sucrose degradation V (sucrose α-glucosidase) (2/5 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (17/26 steps found)
• oxygenic photosynthesis (10/17 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (7/18 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (19/56 steps found)

KEGG Metabolic Maps

• Carbon fixation in photosynthetic organisms
• Fructose and mannose metabolism
• Glycolysis / Gluconeogenesis
• Inositol phosphate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.3.1.1

Protein Sequence (247 amino acids)

>GFF2368 Triosephosphate isomerase (EC 5.3.1.1) (Sphingobium sp. HT1-2)
MSRRKLVVGNWKMNGMRAHLDEVEAIGKLAAQHPAVEVGLCLPATLIMAGSQLKGAAFIG
AQNCHMELSGAYTGSLSAEMLIEAGATWVITGHSERRETRGETNADVAAKSVAAHAAGMK
VILCVGETLAVRDAGDADEVVTAQLLASLPEGASADWLAVAYEPIWAIGTGRIPTLEAIQ
SMHATLRAALASRIGQEQADAMRILYGGSMNGDNAAEIIALPDVDGGLVGGASLSAAKFE
PVIAAAD