Protein Info for GFF2364 in Variovorax sp. SCN45

Annotation: Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00171: Aldedh
  • amino acids 21 to 483 (463 residues), 567.3 bits, E=1.1e-174
• TIGR01780: succinate-semialdehyde dehydrogenase
  • amino acids 31 to 481 (451 residues), 659.4 bits, E=1.4e-202

Best Hits

Swiss-Prot: 54% identical to SLAD_PSEPU: 3-sulfolactaldehyde dehydrogenase (PpSQ1_00395) from Pseudomonas putida

KEGG orthology group: K00135, succinate-semialdehyde dehydrogenase (NADP+) [EC: 1.2.1.16] (inferred from 89% identity to vap:Vapar_1951)

MetaCyc: 53% identical to glutarate semialdehyde dehydrogenase (Pseudomonas aeruginosa)
Glutarate-semialdehyde dehydrogenase. [EC: 1.2.1.20]

Predicted SEED Role

"Aldehyde dehydrogenase B (EC 1.2.1.22)" in subsystem Glycerolipid and Glycerophospholipid Metabolism in Bacteria or Methylglyoxal Metabolism (EC 1.2.1.22)

MetaCyc Pathways

• methylglyoxal degradation IV (3/3 steps found)
• methylglyoxal degradation V (3/3 steps found)
• 4-aminobutanoate degradation III (2/2 steps found)
• L-lactaldehyde degradation (aerobic) (2/2 steps found)
• superpathway of methylglyoxal degradation (6/8 steps found)
• L-lysine degradation X (4/6 steps found)
• L-lysine degradation IV (2/5 steps found)
• lactate biosynthesis (archaea) (2/5 steps found)
• L-lysine degradation I (3/7 steps found)
• L-lysine degradation III (1/6 steps found)
• L-rhamnose degradation II (2/8 steps found)
• superpathway of fucose and rhamnose degradation (4/12 steps found)
• superpathway of L-lysine degradation (12/43 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Glutamate metabolism
• Lysine degradation
• Pyruvate metabolism
• Tyrosine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.16, 1.2.1.22

Use Curated BLAST to search for 1.2.1.16 or 1.2.1.20 or 1.2.1.22

Protein Sequence (489 amino acids)

>GFF2364 Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16) (Variovorax sp. SCN45)
MPLSLSRAELLRSANFIDGEWQAVADRSFDVIDPATGELIARVPDSNAATARAATDAAHA
AFPDWRKTPARQRAQILKRWNDLVLAHQDDLGRLISREQGKPLAEGRGEVAYAASYIEWF
AEQATRANGDVIPAPLPGRRMFALREPVGVVAAITPWNFPAAMIARKIAPALAAGCTVVC
KPAEDTPLTSLALVALAAEAGVPPGVLNIVTASRERTPEVVDAWLDDPRVRKITFTGSTP
VGKHLVRRSADTLKKLSLELGGNAPFIVFEDADIEAAVDGLMAAKFRNGGQTCVCPNRVF
VHEAVHERFAALLVARVAALKVGPASDAASQIGPMINARAVEKIERHVQDAVARGARVLT
GGTRLPALGANYFAPTVLADADATMACACEETFGPVAPLTRFIDEAEVIAQANDTPFGLA
AYFYSRDVRRIWRVADALETGIVGINEGALAAEAAPFGGVKDSGYGREGSVHGLDDYLHT
KYVCQGQLD