Protein Info for GFF2348 in Sphingobium sp. HT1-2

Annotation: Thymidine kinase (EC 2.7.1.21)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00265: TK
  • amino acids 2 to 186 (185 residues), 168.4 bits, E=7.3e-54

Best Hits

Swiss-Prot: 76% identical to KITH_NOVAD: Thymidine kinase (tdk) from Novosphingobium aromaticivorans (strain ATCC 700278 / DSM 12444 / CIP 105152 / NBRC 16084 / F199)

KEGG orthology group: K00857, thymidine kinase [EC: 2.7.1.21] (inferred from 79% identity to sch:Sphch_1250)

MetaCyc: 60% identical to thymidine/deoxyuridine kinase (Escherichia coli K-12 substr. MG1655)
Deoxynucleoside kinase. [EC: 2.7.1.145, 2.7.1.21]; 2.7.1.145,2.7.1.21 [EC: 2.7.1.145, 2.7.1.21]

Predicted SEED Role

"Thymidine kinase (EC 2.7.1.21)" in subsystem Folate Biosynthesis (EC 2.7.1.21)

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleoside salvage (8/9 steps found)
• pyrimidine deoxyribonucleosides salvage (4/5 steps found)
• purine deoxyribonucleosides salvage (4/6 steps found)

KEGG Metabolic Maps

• Drug metabolism - other enzymes
• Pyrimidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.145 or 2.7.1.21

Protein Sequence (192 amino acids)

>GFF2348 Thymidine kinase (EC 2.7.1.21) (Sphingobium sp. HT1-2)
MAKLYFYYSSMNAGKSTTLLQSSFNYQERGMATMLWTAAVDDRFGHGRIASRIGLEAQAH
LFEPGVDMLAAIAAQHDATPLSCVLVDEAQFLTRDQVWQLAAVSDSLGIPVLCYGLRTDF
QGELFEGSAHLLGLADALTEIKTVCDCGRKATMNLRVDASGRAIRQGAQTEIGGNDRYVA
LCRRHFVEQMRG