Protein Info for GFF2345 in Xanthobacter sp. DMC5

Annotation: 2-dehydro-3-deoxy-L-rhamnonate dehydrogenase (NAD(+))

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00106: adh_short
  • amino acids 11 to 201 (191 residues), 210.3 bits, E=3.9e-66
• PF01370: Epimerase
  • amino acids 13 to 213 (201 residues), 29 bits, E=1.5e-10
• PF08659: KR
  • amino acids 13 to 167 (155 residues), 47.2 bits, E=5.2e-16
• PF13561: adh_short_C2
  • amino acids 18 to 246 (229 residues), 202.2 bits, E=2e-63

Best Hits

Swiss-Prot: 39% identical to HCDR_XANP2: 2-(R)-hydroxypropyl-CoM dehydrogenase (xecD) from Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2)

KEGG orthology group: K00059, 3-oxoacyl-[acyl-carrier protein] reductase [EC: 1.1.1.100] (inferred from 88% identity to xau:Xaut_1907)

MetaCyc: 56% identical to 2-dehydro-3-deoxy-L-pentonate 4-dehydrogenase (Herbaspirillum huttiense)
RXN-12095 [EC: 1.1.1.401]; 1.1.1.401 [EC: 1.1.1.401]

Predicted SEED Role

"3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100)" in subsystem Fatty Acid Biosynthesis FASII or mycolic acid synthesis (EC 1.1.1.100)

MetaCyc Pathways

• superpathway of fatty acids biosynthesis (E. coli) (49/53 steps found)
• superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
• palmitate biosynthesis II (type II fatty acid synthase) (29/31 steps found)
• superpathway of unsaturated fatty acids biosynthesis (E. coli) (18/20 steps found)
• superpathway of fatty acid biosynthesis I (E. coli) (15/16 steps found)
• oleate biosynthesis IV (anaerobic) (13/14 steps found)
• (5Z)-dodecenoate biosynthesis I (6/6 steps found)
• palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate) (8/9 steps found)
• fatty acid elongation -- saturated (5/5 steps found)
• gondoate biosynthesis (anaerobic) (4/4 steps found)
• (5Z)-dodecenoate biosynthesis II (5/6 steps found)
• stearate biosynthesis II (bacteria and plants) (5/6 steps found)
• cis-vaccenate biosynthesis (4/5 steps found)
• octanoyl-[acyl-carrier protein] biosynthesis (mitochondria, yeast) (9/12 steps found)
• biotin biosynthesis I (11/15 steps found)
• palmitate biosynthesis III (21/29 steps found)
• 8-amino-7-oxononanoate biosynthesis I (8/11 steps found)
• stearate biosynthesis IV (4/6 steps found)
• anteiso-branched-chain fatty acid biosynthesis (24/34 steps found)
• even iso-branched-chain fatty acid biosynthesis (24/34 steps found)
• odd iso-branched-chain fatty acid biosynthesis (24/34 steps found)
• 8-amino-7-oxononanoate biosynthesis IV (3/5 steps found)
• tetradecanoate biosynthesis (mitochondria) (17/25 steps found)
• L-rhamnose degradation III (4/7 steps found)
• L-arabinose degradation V (2/6 steps found)
• petroselinate biosynthesis (2/6 steps found)
• 2-allylmalonyl-CoA biosynthesis (2/8 steps found)
• streptorubin B biosynthesis (20/34 steps found)
• mycolate biosynthesis (26/205 steps found)
• superpathway of mycolate biosynthesis (27/239 steps found)

KEGG Metabolic Maps

• Biosynthesis of unsaturated fatty acids
• Fatty acid biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.100

Use Curated BLAST to search for 1.1.1.100 or 1.1.1.401

Protein Sequence (250 amino acids)

>GFF2345 2-dehydro-3-deoxy-L-rhamnonate dehydrogenase (NAD(+)) (Xanthobacter sp. DMC5)
MSNAIDLKDRFAIVTGGAQGIGRAVVERFLASGARVAIFDRDTDLVVKTADELARARVMP
VTVDVSRLADVERAVAEVHAEFGRIDILVNNAGVAGANHKTWDYPEEEWKKVMAINLDGP
FFCCRAVAPHMIAQKYGRIVNVASIAGKEGNPNASAYSASKAGVIALTKSLGKELAEYDI
AVNCVTPAAARTAIFDQMSQQHIDYMLSKIPRGRFVQVEEVAATIAFAASADCSFTTGAV
FDLSGGRATY