Protein Info for GFF2343 in Sphingobium sp. HT1-2

Annotation: Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 771 TIGR03591: polyribonucleotide nucleotidyltransferase" amino acids 11 to 692 (682 residues), 1033.3 bits, see alignment E=0 PF01138: RNase_PH" amino acids 14 to 144 (131 residues), 105.9 bits, see alignment E=5.6e-34 amino acids 324 to 457 (134 residues), 93.7 bits, see alignment E=3.3e-30 PF03725: RNase_PH_C" amino acids 147 to 211 (65 residues), 48.8 bits, see alignment E=1.3e-16 amino acids 461 to 530 (70 residues), 29.9 bits, see alignment E=1.1e-10 PF03726: PNPase" amino acids 242 to 321 (80 residues), 46.9 bits, see alignment E=7.9e-16 PF00013: KH_1" amino acids 557 to 614 (58 residues), 45.4 bits, see alignment 1.4e-15 PF00575: S1" amino acids 620 to 691 (72 residues), 73.5 bits, see alignment E=3.6e-24

Best Hits

Swiss-Prot: 85% identical to PNP_NOVAD: Polyribonucleotide nucleotidyltransferase (pnp) from Novosphingobium aromaticivorans (strain ATCC 700278 / DSM 12444 / CIP 105152 / NBRC 16084 / F199)

KEGG orthology group: K00962, polyribonucleotide nucleotidyltransferase [EC: 2.7.7.8] (inferred from 93% identity to sjp:SJA_C1-16260)

Predicted SEED Role

"Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8)" in subsystem Bacterial RNA-metabolizing Zn-dependent hydrolases or Polyadenylation bacterial (EC 2.7.7.8)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (771 amino acids)

>GFF2343 Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8) (Sphingobium sp. HT1-2)
MFDVKKVSIELAGKTLTLETGRIARQADAAVLATYGETVVLCAVTAAKSVKEGQDFFPLT
VHYQEKYSAAGRIPGGFFKRERGATEKETLVSRLIDRPIRPLFPEGFYNEINVIAQVMSF
DGESEPDIVAMIAASAALTLSGVPFMGPIGAARVGYKDGEYQLNPSLDEVKTGELDLVVA
ATSNAVMMVESEAKELSEDVMLGAVLFAHEASKKVVDAIIQLAEKAAKDPWEMAKGDDLG
ALKGKLKDLIGGDIAAAYKLTDKSARSNALNEARAKAKAMFTEDGLDAQTVMAGIKLTKK
LEAEIVRGAILKDGQRIDGRTTTQIRPIEAMVGFLPRTHGSALFTRGETQAICTTTLGTK
DAEQMIDGLTGLHYENFMLHYNFPPYSVGEVGRFGAPGRREVGHGKLAWRALHPVLPTKD
EFPYTIRVLSDITESNGSSSMATVCGGSLSMMDAGVPLKRPVSGIAMGLILEGSDFAVIS
DILGDEDHLGDMDFKVAGTEAGITTMQMDIKVAGITQEIFETALRQAKEGRAHILGEMNK
ALSSTRTELSAHAPRIETIQIDKSKIRDVIGTGGKVIREIVAETGAKVDIDDEGIIKISS
SDTAQIEAAKKWILGIVEEAEVGKIYTGKVVNIVDFGAFVNFMGGKDGLVHVSEMKNERV
EKPTDVVSEGQEVKVKVLEIDPRGKVRLSMRVVDQETGEELEDTRPAREPREPRGDRGDR
GDRGRGPRRDGGDRGGRGGDRGPRRDRGPRSEGKGDDDGSNAGLPDFLTQD