Protein Info for PS417_11940 in Pseudomonas simiae WCS417

Annotation: TonB-dependent receptor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 708 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF07715: Plug" amino acids 54 to 152 (99 residues), 69.3 bits, see alignment E=3.8e-23 TIGR01783: TonB-dependent siderophore receptor" amino acids 56 to 708 (653 residues), 241.7 bits, see alignment E=9.3e-76 PF00593: TonB_dep_Rec_b-barrel" amino acids 275 to 677 (403 residues), 174.8 bits, see alignment E=6e-55

Best Hits

KEGG orthology group: K02014, iron complex outermembrane recepter protein (inferred from 95% identity to pfs:PFLU2593)

Predicted SEED Role

"Outer membrane vitamin B12 receptor BtuB" in subsystem Coenzyme B12 biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See U1TN52 at UniProt or InterPro

Protein Sequence (708 amino acids)

>PS417_11940 TonB-dependent receptor (Pseudomonas simiae WCS417)
MKHLPLLAGLFGCLPVCAWALELAPTTIDGEQTAEPGLALDQSSGMASRLGLSVRETPAS
VAIANRNDIERHGAKTFRDAANTLPGVNASAPPGFGGFVSYRGFTSSQITQMFNGINVAT
GLARPVDAWIYDRVELVGGPSSLINGAGSVGGSLNYVTKLATREEQAAEGQLTYGSYDTA
GMAFGVNHALTEPGADVQHYARLDVSRNTNHSYIDRDQREAWSLAFSLLSDLTPNLSHTL
ALEYQDEHEDSPYWGTPVLNPKVGELKIDKHNRFNNYNVSDGRYEQRTIWARSIIDYRVN
DSTTLKNTLYHLDSQRDYRNLETYQYNADNSAVNRSTAYQVRHQGEQNGNQFELRHDNSL
FGLSTTWSGGFEYKVNSTTNTPLNVPGNSSVDPNNYNPGHFYDIPRTREKFGKDKTNEVT
TQALFVENRLGLTDKLSLLTGLRYDAIDLDVTNHRAITATNPRHFKRSWEPVTGRVGLTY
QFIPSANVYVQYSTAAEQPNTTTQVFDVSTGKQWEVGSKFDYLNGRGSATVAAYKIERKD
FAVTDPQDPTNSIPVGAQSSKGIELASSLRITPRLLAEGNFAWVDAEYDDFNEKIASGAV
VSRKGNTPTNVPKRVGNLWLTYDFAEDWQGGVDARYVAEVYADNANTQTVPAYTLFGTFL
RYKVDSHTSVTGRVRNLTNEVYAEFAHVSPAYYLGAPRTFELAVQTRF