Protein Info for GFF2337 in Xanthobacter sp. DMC5

Annotation: Phosphate-binding protein PstS

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 346 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF12849: PBP_like_2" amino acids 21 to 308 (288 residues), 121.4 bits, see alignment E=3e-39 TIGR00975: phosphate ABC transporter, phosphate-binding protein PstS" amino acids 27 to 335 (309 residues), 393.4 bits, see alignment E=3.5e-122

Best Hits

Swiss-Prot: 51% identical to PSTS_PASMU: Phosphate-binding protein PstS (pstS) from Pasteurella multocida (strain Pm70)

KEGG orthology group: K02040, phosphate transport system substrate-binding protein (inferred from 93% identity to xau:Xaut_0197)

MetaCyc: 50% identical to phosphate ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-27-RXN [EC: 7.3.2.1]; 7.3.2.1 [EC: 7.3.2.1]

Predicted SEED Role

"Phosphate ABC transporter, periplasmic phosphate-binding protein PstS (TC 3.A.1.7.1)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism (TC 3.A.1.7.1)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.3.2.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (346 amino acids)

>GFF2337 Phosphate-binding protein PstS (Xanthobacter sp. DMC5)
VKLTTALGVIAVAGSLFAGSAQAVDFTGAGASFPAPVYQSWGAKFKEKTGSTLNYQSIGS
GGGQNQIINRTVDFGASDATVPGEKLAANNLLQFPTVIGSVVATVNLDGITTNQLKLTGP
VLADIYLGKITKWNDKAIADLNPGVTLPANAIVPVYRSDSSGTTSIFTAYLSAVSPDWKS
KVGSATSVQWPTGAGAKGNEGVTGTVKNTKGGIGYVEFIYAAANKLVVTQLQNKAGKFVS
PEVAGFQAAAASADWKGAKDFAASMIDAPAPEAWPIVSPTYILLPKNPSNVEASREVMKF
FEWAYTKEGADIAEGLHYIPLPAAVVEQVKAAFKSEVKGPDGKPVL