Protein Info for PS417_11855 in Pseudomonas simiae WCS417

Updated annotation (from data): Myo-inosose-2 dehydratase (EC 4.2.1.44)
Rationale: Specifically important for utilizing m-Inositol. Automated validation from mutant phenotype: the predicted function (MYO-INOSOSE-2-DEHYDRATASE-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: xylose isomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 295 TIGR04379: myo-inosose-2 dehydratase" amino acids 4 to 290 (287 residues), 379 bits, see alignment E=9.1e-118 PF01261: AP_endonuc_2" amino acids 35 to 274 (240 residues), 95.9 bits, see alignment E=1.6e-31

Best Hits

Swiss-Prot: 41% identical to IOLE_GEOTN: Inosose dehydratase (iolE) from Geobacillus thermodenitrificans (strain NG80-2)

KEGG orthology group: K03335, inosose dehydratase [EC: 4.2.1.44] (inferred from 97% identity to pfs:PFLU2576)

Predicted SEED Role

"Inosose dehydratase (EC 4.2.1.44)" in subsystem Inositol catabolism (EC 4.2.1.44)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.44

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See U1TM99 at UniProt or InterPro

Protein Sequence (295 amino acids)

>PS417_11855 Myo-inosose-2 dehydratase (EC 4.2.1.44) (Pseudomonas simiae WCS417)
MPAIRIGINPISWSNDDLPALGGETPLSTALSEGKDIGYEGFELNGKFPKDAKGVGDVLR
PYDLALVSGWYSSRLARRSVAEEIEAIAGHVELLKHNGATVLVYGEVADSIQGSRIRLIE
RPRFHSEQAWQDYADKLTELARFTLSQGVRLAYHHHMGAYVESPEDIDQLMRRTGPEVGL
LFDSGHCYMGGGEPIEVLRKHIDRVCHVHFKDVRKAVVQLARNQMWSFPDCIVNGTFTVP
GDGDIDFAELLDVLLAAQYEGWLVVEAEQDPAVAPSYIYAKKGYDTLRALLNERT