Protein Info for PS417_11790 in Pseudomonas simiae WCS417

Annotation: ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 362 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF01547: SBP_bac_1" amino acids 38 to 295 (258 residues), 57.7 bits, see alignment E=4.9e-19 PF13416: SBP_bac_8" amino acids 40 to 326 (287 residues), 112.9 bits, see alignment E=6.3e-36 PF13343: SBP_bac_6" amino acids 74 to 313 (240 residues), 51.3 bits, see alignment E=2.8e-17

Best Hits

Swiss-Prot: 64% identical to SPUE_PSEAE: Spermidine-binding periplasmic protein SpuE (spuE) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02055, putative spermidine/putrescine transport system substrate-binding protein (inferred from 95% identity to pfs:PFLU2564)

MetaCyc: 57% identical to putrescine ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-25-RXN [EC: 7.6.2.11, 7.6.2.16]

Predicted SEED Role

"Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2)" in subsystem Polyamine Metabolism (TC 3.A.1.11.2)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.6.2.11 or 7.6.2.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U5Q8 at UniProt or InterPro

Protein Sequence (362 amino acids)

>PS417_11790 ABC transporter substrate-binding protein (Pseudomonas simiae WCS417)
MRLVKQLFPLALVAAFSSASQAAPTVSVYNWTDYIGETTLADFQAKTGIKPIYDVFDSNE
TLEGKLLAGRTGYDVVVPSNHFLARQVKAGAFLKLDRAQLPNFKNLDPKLLKLLEKNDPG
NAHSVPYLWGTNGIGYNVDKVKEVLGIDAIDSWAVLFEPENIKKLNQCGVAFLDSADELF
PAILNYMGKDPRSENPEDYKQAEAKLLTLRPYITYFHSSKYISDLANGNICVAFGYSGDV
FQAANRAKEAKNGVKIAYSIPKEGSNLWFDLLAIPADASHPKEAHAFINYLLDPQVIAKV
SASVGYANANPAAKAFMDPELVNNPEVYPSQDVLDKLYISTTPTPATMRLMTRAWSKVKT
NK