Protein Info for PS417_11755 in Pseudomonas simiae WCS417

Annotation: diacetyl reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 59% identical to BUDC_KLEPN: Diacetyl reductase [(S)-acetoin forming] (budC) from Klebsiella pneumoniae

KEGG orthology group: K03366, (R,R)-butanediol dehydrogenase / diacetyl reductase [EC: 1.1.1.303 1.1.1.4] (inferred from 75% identity to pin:Ping_2541)

MetaCyc: 59% identical to acetoin reductase subunit (Klebsiella pneumoniae)
RXN-11032 [EC: 1.1.1.304]; 1.1.1.- [EC: 1.1.1.304]

Predicted SEED Role

"2,3-butanediol dehydrogenase, S-alcohol forming, (S)-acetoin-specific (EC 1.1.1.76)" in subsystem Acetoin, butanediol metabolism (EC 1.1.1.76)

MetaCyc Pathways

pyruvate fermentation to (R)-acetoin I (3/3 steps found)
pyruvate fermentation to (S)-acetoin (3/3 steps found)
superpathway of 2,3-butanediol biosynthesis (5/6 steps found)
superpathway of (R,R)-butanediol biosynthesis (4/5 steps found)
(R,R)-butanediol biosynthesis (1/1 steps found)
(S,S)-butanediol biosynthesis (1/1 steps found)
(R,R)-butanediol degradation (1/1 steps found)
(S,S)-butanediol degradation (1/1 steps found)
meso-butanediol biosynthesis I (1/1 steps found)

KEGG Metabolic Maps

Butanoate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.303 or 1.1.1.304 or 1.1.1.4 or 1.1.1.76

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UK49 at UniProt or InterPro

Protein Sequence (259 amino acids)

>PS417_11755 diacetyl reductase (Pseudomonas simiae WCS417)
MAITGKVALVTGAGQGIGRAIALRLAQDGADIALVDINGARLEAVASEVVALGRQASVFV
ADVSKREQVVAAVEHAHQTLGGFDIIVNNAGVAQIDSLLEVSPEQVERTLGINVQGVLWG
IQAAGKKFKALKQKGKIINACSIAGHEGFALLGVYSATKFAVRALTQAAAKELASAGITV
NAYCPGVVGTDMWVEIDKRMAEITGADVGATYKKYVDGIALGRAETPEDVAGLVSYLAGP
DSDYMTGQSPLIDGGLVYR