Protein Info for GFF2302 in Xanthobacter sp. DMC5

Annotation: Polyribonucleotide nucleotidyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 726 TIGR03591: polyribonucleotide nucleotidyltransferase" amino acids 11 to 694 (684 residues), 1077.3 bits, see alignment E=0 PF01138: RNase_PH" amino acids 16 to 144 (129 residues), 113.6 bits, see alignment E=2.2e-36 amino acids 327 to 459 (133 residues), 104.2 bits, see alignment E=1.8e-33 PF03725: RNase_PH_C" amino acids 147 to 210 (64 residues), 58 bits, see alignment E=1.9e-19 amino acids 463 to 531 (69 residues), 27.9 bits, see alignment E=4.5e-10 PF03726: PNPase" amino acids 240 to 323 (84 residues), 68.6 bits, see alignment E=1.3e-22 PF00013: KH_1" amino acids 559 to 616 (58 residues), 38.4 bits, see alignment 2.1e-13 PF00575: S1" amino acids 621 to 693 (73 residues), 66.6 bits, see alignment E=5.3e-22

Best Hits

Swiss-Prot: 89% identical to PNP_AZOC5: Polyribonucleotide nucleotidyltransferase (pnp) from Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571)

KEGG orthology group: K00962, polyribonucleotide nucleotidyltransferase [EC: 2.7.7.8] (inferred from 89% identity to azc:AZC_0063)

Predicted SEED Role

"Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8)" in subsystem Bacterial RNA-metabolizing Zn-dependent hydrolases or Polyadenylation bacterial (EC 2.7.7.8)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (726 amino acids)

>GFF2302 Polyribonucleotide nucleotidyltransferase (Xanthobacter sp. DMC5)
MFDIHREELDWGGTTLTLETGKMARQADGSVLATYGDTKVLATVVSAKEPKPGQDFFPLT
VNYQEKTYAAGRIPGGYFKREGRPSEKETLVSRLIDRPIRPLFPEGYKCDTQVVVTVLAH
DLENDPDVVALVAASAALTLSGIPFMGPVGAARVGFIDNEYVLNPTVDEVKESSLDLVVA
GTGDAVLMVESEAKELPEEIMLGAVMFGHRHFQPVIEAIIKLAEKAAKEPRTFAPLDVSE
IEAKVKEIAEHDLRAAYKIKQKQDRYAAVGAAKSKVKKYYEELAIGGTSVPNPQVVSEVF
KALEAKIVRWNILDDGIRIDGRDVRTVRPIVSEVGILPRAHGSALFTRGETQALVVATLG
TGEDEQFIDSLEGTYKEHFLLHYNFPPFSVGETGRMGSPGRREIGHGKLAWRAIRPMLPA
KHEFPYTLRVVSEILESNGSSSMATVCGTSLALMDAGVPLRRPVAGIAMGLILEDEKFAV
LSDILGDEDHLGDMDFKVAGTEQGVTSLQMDIKIAGITEEIMKVALTQAKDGRVHILGEM
SKALTTARAELGEHAPRIEVMKIAVDKIREVIGSGGKVIREIVEKTGAKINIEDDGTIKI
ASASGDSIKAAINWIKSIASEPEVGQIYEGTVVKVVDFGAFVNFFGSKDGLVHVSQMANE
RVAKPSDVVKEGDKVKVKLMGFDERGKTRLSMKVVDQTTGEDLEAKAKAEREAGRSASEG
EAGAAE