Protein Info for GFF2296 in Sphingobium sp. HT1-2

Annotation: Aconitate hydratase (EC 4.2.1.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01341: aconitate hydratase 1
  • amino acids 21 to 887 (867 residues), 1348.9 bits, E=0
• PF00330: Aconitase
  • amino acids 71 to 561 (491 residues), 561.6 bits, E=1.6e-172
• PF00694: Aconitase_C
  • amino acids 693 to 816 (124 residues), 141.1 bits, E=2.8e-45

Best Hits

Swiss-Prot: 64% identical to ACNA_BRADU: Aconitate hydratase A (acnA) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: K01681, aconitate hydratase 1 [EC: 4.2.1.3] (inferred from 66% identity to azc:AZC_4461)

Predicted SEED Role

"Aconitate hydratase (EC 4.2.1.3)" in subsystem Propionate-CoA to Succinate Module or Serine-glyoxylate cycle or TCA Cycle (EC 4.2.1.3)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
• superpathway of glyoxylate bypass and TCA (11/12 steps found)
• TCA cycle I (prokaryotic) (9/10 steps found)
• TCA cycle III (animals) (9/10 steps found)
• glyoxylate cycle (6/6 steps found)
• mixed acid fermentation (13/16 steps found)
• partial TCA cycle (obligate autotrophs) (7/8 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (17/22 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• TCA cycle II (plants and fungi) (7/9 steps found)
• TCA cycle V (2-oxoglutarate synthase) (7/9 steps found)
• TCA cycle VI (Helicobacter) (7/9 steps found)
• TCA cycle VII (acetate-producers) (7/9 steps found)
• nitrogen remobilization from senescing leaves (6/8 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (6/9 steps found)
• methylaspartate cycle (13/19 steps found)
• ethene biosynthesis V (engineered) (17/25 steps found)
• reductive TCA cycle I (6/11 steps found)
• reductive TCA cycle II (6/12 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glyoxylate and dicarboxylate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.3

Use Curated BLAST to search for 4.2.1.3

Protein Sequence (889 amino acids)

>GFF2296 Aconitate hydratase (EC 4.2.1.3) (Sphingobium sp. HT1-2)
MTATGQDTLGTRDTLNVGGKDIAYYSLEKAAAKLGDVSRLPFSMKVLLENLLRFEDGVTV
TTDDIKAIVDWQNDKGKAEREIQYRPARVLLQDFTGVPCVVDLAAMRDAMNALGADASKI
NPQVPVHLVIDHSVMVDEFGTPRAFQDNMEIEYQRNMERYDFLKWGSKSLANFYAVPPGT
GICHQVNLENIAQGVWSSEGADGSTIAYPDTCVGTDSHTTMVNGLGVLGWGVGGIEAEAA
MLGQPVSMLIPEVVGFKFTGNLKEGVTATDLVLTCTQMLRARGVVGRFVEYFGPGLATLS
LADRATLANMAPEYGATCGFFGIDDKTLDYMRLTGRSDENIALVEAYAKQQGFWIDPSVE
PIFTDTLELDLSTVVPSLAGPKRPQDRVALPQVDDVFNADMVNTYKKAQTRVPVEGKDYD
IGDGDVTIAAITSCTNTSNPNVLIAAGLVAKKANELGLKPKPWVKTSLAPGSQVVTDYLE
KAGLQAHLDAIGFNLVGYGCTTCIGNSGPLAEPISKAINENGLVAAAVISGNRNFEGRVS
PDVRANFLASPPLVVAYALKGTVIEDFITTPIGTSKDGEAVYLKDIWPTNDEVATTLAGA
VDREMFRARYANVYKGDAHWQAIEVTGSDTYKWRAGSTYVANPPYFEGLTMTPAPVTDII
GAMPLAIFGDSITTDHISPAGSIKATSPAGKWLSEHQVAQADYNSYGSRRGHHEVMMRGT
FANIRIKNLMLDGVEGGMTSYQGEVMPIYDAAMKHKADGTPLVVIGGKEYGTGSSRDWAA
KGTNLLGVRAVIVESFERIHRSNLVGMGVLPLVFKDGQTRDTLGLKGDDSFTITGVADLK
PRQDVEVQVTRADGSTFSFTALCRIDTVNELEYFLNGGILQYVLRKLAA