Protein Info for GFF2295 in Xanthobacter sp. DMC5

Annotation: Translation initiation factor IF-2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 785 TIGR00487: translation initiation factor IF-2" amino acids 199 to 785 (587 residues), 773.8 bits, see alignment E=1.3e-236 PF04760: IF2_N" amino acids 206 to 257 (52 residues), 53 bits, see alignment 6.8e-18 TIGR00231: small GTP-binding protein domain" amino acids 285 to 440 (156 residues), 106.5 bits, see alignment E=1.2e-34 PF01926: MMR_HSR1" amino acids 287 to 393 (107 residues), 41.9 bits, see alignment E=2.9e-14 PF00009: GTP_EFTU" amino acids 287 to 443 (157 residues), 117 bits, see alignment E=2.5e-37 PF22042: EF-G_D2" amino acids 459 to 537 (79 residues), 98.8 bits, see alignment E=4.7e-32 PF11987: IF-2" amino acids 564 to 675 (112 residues), 140.5 bits, see alignment E=7e-45

Best Hits

Swiss-Prot: 73% identical to IF2_NITHX: Translation initiation factor IF-2 (infB) from Nitrobacter hamburgensis (strain DSM 10229 / NCIMB 13809 / X14)

KEGG orthology group: K02519, translation initiation factor IF-2 (inferred from 73% identity to nha:Nham_0031)

Predicted SEED Role

"Translation initiation factor 2" in subsystem NusA-TFII Cluster or Translation initiation factors eukaryotic and archaeal or Universal GTPases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (785 amino acids)

>GFF2295 Translation initiation factor IF-2 (Xanthobacter sp. DMC5)
VVLRTLTEEERNARASALADARVRAVEEQRMAEERRAAEEEARRRAERERAERAEREAAE
ARKREDEARRAQEEDKKRKAEQEARKRFGGEDQGASARPAGTTTARPLTPRPAGTTAPTE
GGSEEEERRGGGAVRRGPARPVAPVKVPRAPAGQEKQRGRLTLVTAQSGEEERQRSVASF
RRRTQRMTGHRQQESKEKIAREVILPETITIQELANRMSERAVDVIRMLMKQGQMVKITD
VIDADTAELIAEDLGHTVRRVSESDVEEGLFDTPDAPETLLPRPAVVTIMGHVDHGKTSL
LDAIRKANVVSGEAGGITQHIGAYQVTAPSGSKITFIDTPGHAAFTAMRARGAKVTDIVV
LVVAADDGVMPQTVEAINHARAARVPLIVAINKIDKPGAKPERVRSELLQYEVQVESMGG
ETLEVEVSAKEHLNLDKLLEAIALQSELLDLRANPNRDAEGTVVEAKLDRGRGPVATVLV
QRGTLHVGDIVVAGAEWGRVRALISDTGATVDAAGPSFPVEVLGFNGTPEAGDRLAVVEN
EARAREITDYRQRQKREKAAARSATIRGSLEQMMSQVKASGRKEFPLIIKGDVSGSVEAI
IGALEKVGNDEVQARIIHSGAGGINESDVTLAETSGAAIIAFNVRANKEARDAADRAGIE
IRYYNIIYDLVDDVKKAMSGLLAPINRETMLGNALIKEIFLVSKVGKVAGCQVTDGIVEK
GQHVRLIRDNVVIHEGKLATLNRFKDAVNTVNAGQDCGMSFENYQDMRAGDVIECYRVEV
IQRSL