Protein Info for GFF2286 in Xanthobacter sp. DMC5

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01849: polyhydroxyalkanoate depolymerase, intracellular
  • amino acids 24 to 428 (405 residues), 613.2 bits, E=9.2e-189
• PF06850: PHB_depo_C
  • amino acids 229 to 429 (201 residues), 325.5 bits, E=5.9e-102

Best Hits

KEGG orthology group: K05973, poly(3-hydroxybutyrate) depolymerase [EC: 3.1.1.75] (inferred from 87% identity to xau:Xaut_0304)

Predicted SEED Role

"Intracellular PHB depolymerase (EC 3.1.1.-)" in subsystem Polyhydroxybutyrate metabolism (EC 3.1.1.-)

KEGG Metabolic Maps

• 2,4-Dichlorobenzoate degradation
• Alkaloid biosynthesis II
• Ascorbate and aldarate metabolism
• Butanoate metabolism
• Glycerophospholipid metabolism
• Retinol metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.-

Use Curated BLAST to search for 3.1.1.- or 3.1.1.75

Protein Sequence (474 amino acids)

>GFF2286 hypothetical protein (Xanthobacter sp. DMC5)
MAIGEFASATRLPRQGDIGLSTPLYWMYEMGYAALNPSRAMADASRLFLKNPINPVSHTT
IGKSMAAACELFERTTRRYGKPDWGITHTSAGGQTVPIHVNTVWERPFCRLLHFERAFEY
PPRRPQPRLLIVAPVSGHHATLLRGTVEAMLPTHDVYVTDWIDAKLVPMSEGRFSLSDYV
DYLISIFHRMGGNCHVMAVCQPAVPVMMAIARMEAEEDPFVPRSMVLMGGPIDTREAPTA
VNTLAQDRGIEWFRRNVITTVPWPHPGALREVYPGFLQLNGFMSMNLDRHLQAHWDLFRH
LVQGDGDSAEKHRDFYDEYLAVMDLTADFYLETVENVFITHALPKGEIMHHSQVVKPDAI
HRVALMTVEGEKDDISGIGQTKAAHKLCPRIPEDMRAHWEQPGVGHYGVFNGSRFRSEIA
PRISDFILSQEFRKDGRARPMPDRPAPVAAGEEEPDVFALNKNVTALYPRIAPT