Protein Info for PS417_11635 in Pseudomonas simiae WCS417

Annotation: 3-carboxy-cis,cis-muconate cycloisomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00206: Lyase_1
  • amino acids 19 to 297 (279 residues), 182.6 bits, E=1.3e-57
• PF10397: ADSL_C
  • amino acids 365 to 440 (76 residues), 70.2 bits, E=1.6e-23

Best Hits

Swiss-Prot: 49% identical to TAD1_USTMD: Trans-aconitate decarboxylase 1 (TAD1) from Ustilago maydis

KEGG orthology group: K01857, 3-carboxy-cis,cis-muconate cycloisomerase [EC: 5.5.1.2] (inferred from 73% identity to dac:Daci_1439)

MetaCyc: 49% identical to trans-aconitate decarboxylase (Ustilago maydis)
RXN-19902 [EC: 4.1.1.113]

Predicted SEED Role

"3-carboxy-cis,cis-muconate cycloisomerase (EC 5.5.1.2)" in subsystem Protocatechuate branch of beta-ketoadipate pathway (EC 5.5.1.2)

MetaCyc Pathways

• aromatic compounds degradation via β-ketoadipate (9/9 steps found)
• protocatechuate degradation II (ortho-cleavage pathway) (4/4 steps found)
• toluene degradation III (aerobic) (via p-cresol) (8/11 steps found)
• itaconate biosynthesis II (1/3 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (21/35 steps found)
• superpathway of aerobic toluene degradation (12/30 steps found)

KEGG Metabolic Maps

• Benzoate degradation via hydroxylation

Isozymes

Compare fitness of predicted isozymes for: 5.5.1.2

Use Curated BLAST to search for 4.1.1.113 or 5.5.1.2

Sequence Analysis Tools

See A0A1N7U5N2 at UniProt or InterPro

Protein Sequence (451 amino acids)

>PS417_11635 3-carboxy-cis,cis-muconate cycloisomerase (Pseudomonas simiae WCS417)
MSSTVFDSALFRDMFGTAEMRGVFSDKALIERYIEVEVALARAEARCGVIPTDAAEQIAT
LSQYEALDLALMQHETEIVGYPILPLVEQLSKICGEAGRYVHWGATTQDIMDTAVVLQVR
AALAIVERDIQTVRGLLAGLAERYRDTPMAGRTHLQHALPITFGYKCAVWLSMFDRHAER
LVELRPRVEIGQFAGAAGTLASLGDKGLEVQEALMSELGLGVPQATWHVARDGLAETLNF
LGLVTGSLGKIALDIMMMMTSELGEVYEPFVKGRGASSTMPQKRNPISCELMYAAAKGVR
QHAGLMLDAMIQDFERSTGPWQAEWIAIPEAFALSAASLGQATFMLAGLEVRPERMRKNL
DMTQGLIVAEAVMMGLAPALGRQVAHDVVYAACRMANDQGTSLLDALLAQGEATAQLDLA
ELQRLTDPANYLGLAPQMVDIALARQGAVKR