Protein Info for GFF2282 in Variovorax sp. SCN45

Annotation: Trans-feruloyl-CoA synthase (EC 6.2.1.34)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 617 PF00501: AMP-binding" amino acids 47 to 431 (385 residues), 171.3 bits, see alignment E=3e-54 PF23562: AMP-binding_C_3" amino acids 477 to 604 (128 residues), 24.6 bits, see alignment E=2.9e-09

Best Hits

KEGG orthology group: K12508, feruloyl-CoA synthase [EC: 6.2.1.34] (inferred from 91% identity to vap:Vapar_4403)

Predicted SEED Role

"Trans-feruloyl-CoA synthase (EC 6.2.1.34)" (EC 6.2.1.34)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.34

Use Curated BLAST to search for 6.2.1.34

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (617 amino acids)

>GFF2282 Trans-feruloyl-CoA synthase (EC 6.2.1.34) (Variovorax sp. SCN45)
MSAVRYRPLVFGVTRAVLRDGAPGTQYLSAETQLAPYRERMTDRLAYWAERTPDRTFIAR
RERLADGSTGDWVRVSYAQALKEARGIGQALLDRGLSAERPVAILSENGIEHALLALGCL
YAGVPYCPVSPPYSLVSQDFEKLRHVFDTLTPGLVFASDAARFAKAIAAVVPADTEVVLA
QGTLEGRPTTAFDALASTPATPAIDEAMRATGPDTITKFLFTSGSTKMPKAVINTHRMWC
ANQQQLRQSIPALGDEPPVLVDWLPWNHTFGGNHNVGIVLDNGGTLYVDDGKPTPAGMAE
TLRNLREIAPTIYFNVPTGFEAIAHAMETDAVLRRNLLSRVKMFFYSGAALSQPVWDSLH
RTQESEVGERIVMGTGLGMTESGPFALYVTGPDVKSGDVGLPAPGIELKLIEVDGKTEVR
YRGPNITPGYWRAPEATAEAFDEEGFFSTGDAVKWIDDADIHRGLRFDGRIAEDFKLATG
TFVSVGPMRAKIIAAGSPYVQDAVLTGINLKEVGALIFPTQKVRQLAGLPADATMRQVLE
SAPVQAHFQQVVDTLAAGATGSANRIARLHLMAEPPSIDKGEVTDKGSINQRAVLKHRAE
LADAMHGAALPFTLKPR