Protein Info for PS417_01155 in Pseudomonas simiae WCS417

Annotation: glutamate--cysteine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 527 transmembrane" amino acids 193 to 212 (20 residues), see Phobius details TIGR01434: glutamate--cysteine ligase" amino acids 7 to 516 (510 residues), 751.9 bits, see alignment E=1.6e-230 PF04262: Glu_cys_ligase" amino acids 10 to 380 (371 residues), 555.8 bits, see alignment E=1.8e-171

Best Hits

Swiss-Prot: 90% identical to GSH1_PSEPF: Glutamate--cysteine ligase (gshA) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K01919, glutamate--cysteine ligase [EC: 6.3.2.2] (inferred from 98% identity to pfs:PFLU0256)

Predicted SEED Role

"Glutamate--cysteine ligase (EC 6.3.2.2)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle (EC 6.3.2.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UB87 at UniProt or InterPro

Protein Sequence (527 amino acids)

>PS417_01155 glutamate--cysteine ligase (Pseudomonas simiae WCS417)
MSELLNRRLALLGKREHLSLLEQCLHGIERECLRVTGEGRLAQTPHPEALGAALTHEQIT
TDYSESLLEFITPALPNPADTLSSLDKIHRFAYTKLGSEYLWSPSMPCPLPAEEDIPIAY
YGTSNIGQLKYVYRKGLALRYGKTMQCIAGIHYNFSLPEQLWPLLKETEGFVGTDRDYQS
NAYIALIRNFRRYSWLLMYLFGASPALDAGFLRGRSHQLEVLDADTLYLPYATSLRMSDL
GYQSNAQAGLTPCYNDLSSYTDSLREAVATPYAPYVEVGTHKDGEWVQLNTNILQIENEY
YSNIRPKRVTYTGERPIQALMARGIQYIEVRCLDINPFLPMGIDLPESRFLDAFLLYCAL
NDSPLFANNECGNATSNFLSVVKEGRRPGLQLQRQGQAVDMKEWAAELLEQIAPLAALLD
EAHGITEHSQALDAQLAKVEDPSLTPSAQVLAAMAERKESFAQFSLHQSQLHAEHFRKEP
LPAEEQARFEELARQSLVQQAELEQNEVGDFDVFVGSYQASILAISN