Protein Info for GFF2276 in Variovorax sp. SCN45

Annotation: Chlorogenate esterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 579 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details PF07519: Tannase" amino acids 87 to 574 (488 residues), 444.6 bits, see alignment E=5e-137

Best Hits

KEGG orthology group: K09252, feruloyl esterase [EC: 3.1.1.73] (inferred from 69% identity to vap:Vapar_5482)

Predicted SEED Role

"Chlorogenate esterase" in subsystem Phenylpropanoid compound degradation

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.73

Use Curated BLAST to search for 3.1.1.73

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (579 amino acids)

>GFF2276 Chlorogenate esterase (Variovorax sp. SCN45)
MTRQQQRHLHLGLCASALGVAALLAACAARPPAPHAPLAAARPGTLQACTDLATRAALPG
TVYTSVTDVPAGTVTVGGVAMAAPAHCLVQGRMNERTSPVDNQRYAVGFEMRLPVAWNGR
FFYQANGGLDGVVLPALGGIGGGGPRSSALQMGFAVISSDAGHAGSMGPRFGLDPQARLD
YGYNAVAQLTPMAKNLISKAYGRGPDRSYFGGCSNGGRHAMVAAVRSAQQYDGILAGDPG
FNLPKAAVAQLYGAQQYAAVASAKTPAGQPDLQSAFTPAELKLVADKVLARCDALDGLAD
GIVSNVRACRTRFDIQRDVPTCAAGTRDGMCLSAPQKNALANVFKGARNSAGQPLYSSFP
FDAGVSGSNWREWKFASPPTRDAGAVAFIFSTPPLADRPQGLPFALGFDMDRDAPRIAAT
DGVFRESALSFMTPPDAANLSALRDRGGRMIVYHGTSDPVFSSDDTAAWYERVQAANGSD
ASAFARYFPVPGMNHCSGGPATDQFDMLRALVDWVEQGKAPASVTASARGAGTPVPNAEV
PANWSPQRTRPLCPYPQVATYTGGDTERASSFACRPPSP