Protein Info for GFF227 in Xanthobacter sp. DMC5

Annotation: Sodium-potassium/proton antiporter ChaA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 365 transmembrane" amino acids 7 to 27 (21 residues), see Phobius details amino acids 36 to 59 (24 residues), see Phobius details amino acids 71 to 93 (23 residues), see Phobius details amino acids 105 to 127 (23 residues), see Phobius details amino acids 139 to 157 (19 residues), see Phobius details amino acids 170 to 190 (21 residues), see Phobius details amino acids 213 to 236 (24 residues), see Phobius details amino acids 256 to 276 (21 residues), see Phobius details amino acids 288 to 312 (25 residues), see Phobius details amino acids 324 to 341 (18 residues), see Phobius details amino acids 346 to 364 (19 residues), see Phobius details PF01699: Na_Ca_ex" amino acids 41 to 192 (152 residues), 53.6 bits, see alignment E=1.3e-18 amino acids 220 to 363 (144 residues), 40.2 bits, see alignment E=1.7e-14

Best Hits

KEGG orthology group: K07300, Ca2+:H+ antiporter (inferred from 76% identity to xau:Xaut_3493)

Predicted SEED Role

"Calcium/proton antiporter"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (365 amino acids)

>GFF227 Sodium-potassium/proton antiporter ChaA (Xanthobacter sp. DMC5)
MGKRFAVSAYVLPASGILLALTFFLWPSLSPAASGFLPGVLAFGSIALLCGSAFSAVGHA
DVVEARLGQPWGTLVLTLSVTVIEVSVMASMMLNGENNPTLARESVLSVLMIVNAGIVGL
CLLLGSIRHREQTIQQQGISGYLSATIALGVMLLILPSETRAGASGIFSPFQLIYMSAIA
LALYAAFLYMQTVRYREHFNAQPEGHAEAPPELGAFILAIVMLLASLGAVVALSGWVAMS
FEDFISQFALRDPDAVIGALVATLILLPEGMSAVRAAARNHLQHSLNIALGSALATVSLT
IPAMAFISVMIGRPMVLGLDNEDRTMLLLTFVLAVLSFGTGKTNALNGVIHLIVFCVYVM
LLFAP