Protein Info for GFF2267 in Xanthobacter sp. DMC5
Annotation: Phosphoenolpyruvate carboxylase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 67% identical to CAPP_RHOPA: Phosphoenolpyruvate carboxylase (ppc) from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
KEGG orthology group: K01595, phosphoenolpyruvate carboxylase [EC: 4.1.1.31] (inferred from 84% identity to xau:Xaut_0318)Predicted SEED Role
"Phosphoenolpyruvate carboxylase (EC 4.1.1.31)" in subsystem Fermentations: Mixed acid or Pyruvate metabolism I: anaplerotic reactions, PEP (EC 4.1.1.31)
MetaCyc Pathways
- ethene biosynthesis V (engineered) (20/25 steps found)
- nitrogen remobilization from senescing leaves (7/8 steps found)
- partial TCA cycle (obligate autotrophs) (7/8 steps found)
- CO2 fixation into oxaloacetate (anaplerotic) (2/2 steps found)
- C4 photosynthetic carbon assimilation cycle, NAD-ME type (8/11 steps found)
- C4 photosynthetic carbon assimilation cycle, NADP-ME type (5/7 steps found)
- mixed acid fermentation (11/16 steps found)
- C4 photosynthetic carbon assimilation cycle, PEPCK type (9/14 steps found)
- formaldehyde assimilation I (serine pathway) (7/13 steps found)
- gluconeogenesis II (Methanobacterium thermoautotrophicum) (10/18 steps found)
- Methanobacterium thermoautotrophicum biosynthetic metabolism (25/56 steps found)
KEGG Metabolic Maps
- Carbon fixation in photosynthetic organisms
- Pyruvate metabolism
- Reductive carboxylate cycle (CO2 fixation)
Isozymes
No predicted isozymesUse Curated BLAST to search for 4.1.1.31
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (926 amino acids)
>GFF2267 Phosphoenolpyruvate carboxylase (Xanthobacter sp. DMC5) MTNLALSSGLAPRAEDVKDQPLRDDIRLLGRILGDTVRDQEGDEVFDLVERIRQLSIRFH RDDDQAARDELGTLLGALPADRSLDTIRAFSYFSHLANIAEDQHHIRRNRAHAIAGSAPR AGSLAYAFSRAEQMGIDRETLAHFFSGARMSPVLTAHPTEVRRKSTLNREMEIAAELDRR ERVVPTPQEREEGEEILRRAVLTLWQTALLRRIKLTVLDEVANGLSYYDYTFLTEVPRLY CAIEDRLAEAGDEVQLPSFLRIGSWIGGDRDGNPFVTAEVLKETVRLHRDRILAHYEEEL VALGAELSLAQRLVTVSDALTELAARSPDRSQEHREEPYRLALAYVLERLRQTARKLRGE PLATLEALAPYATAQEFRADLDVIHDSLVANGSKVLARGRLRLLRRAADCFGFHLACIDL RQNSDVHERVVAELLEKAVPGTNYAGLDEEARIALLLKELATPRPLASPFIAYSEETCSE LDILREAAAAHHALGREVIPNAIISKAEGVSDLLEVALLLKEVGLVASDGTGGLASEVNI IPLFETISDLKACAKVMDRAFSLPAYRKLVASRGAEQEVMLGYSDSNKDGGFVTSGWELY KAEIGLIEVFREHKVRLRLFHGRGGSVGRGGGPSYDAILAQPGGAVNGQIRITEQGEIIA SKYSNPDVGRRNLEILVSATLEASLLQPQNKAPRTEFLTAMEDISDTAFAAYRNLVYETE GFEDYFWSSTVINEIATLNIGSRPASRAKTRSIEKLRAIPWVFSWAQCRLMLPAWYGFGT AVEAFVARRPDYGLSFLKAMQHEWPFFRTLLSNMDMVLAKSSLAIASRYAELVPDVELRT AIFGRIRAEYELTLKHLLAIMDQQKLLEGNPLLDRSIRNRFPYLDPLNHLQVELLRQHRA SPGDDKVLHGIQLSINGISAGLRNSG