Protein Info for GFF2243 in Xanthobacter sp. DMC5

Annotation: Ribosomal RNA small subunit methyltransferase I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 323 transmembrane" amino acids 155 to 174 (20 residues), see Phobius details TIGR00096: 16S rRNA (cytidine(1402)-2'-O)-methyltransferase" amino acids 44 to 309 (266 residues), 237.4 bits, see alignment E=1e-74 PF00590: TP_methylase" amino acids 44 to 242 (199 residues), 118.3 bits, see alignment E=4.7e-38 PF23016: RsmI_C" amino acids 267 to 311 (45 residues), 47.7 bits, see alignment 1.1e-16

Best Hits

Swiss-Prot: 56% identical to RSMI_RHILO: Ribosomal RNA small subunit methyltransferase I (rsmI) from Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099)

KEGG orthology group: K07056, (no description) (inferred from 81% identity to xau:Xaut_0340)

Predicted SEED Role

"rRNA small subunit methyltransferase I" in subsystem Heat shock dnaK gene cluster extended

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (323 amino acids)

>GFF2243 Ribosomal RNA small subunit methyltransferase I (Xanthobacter sp. DMC5)
MVGVMTTPHRPHPHSRPEGRAAGVVKTFLLAGSTLPAPGLSPGLHVVATPIGNLADVTVR
ALETLAAADVIACEDTRISRRLLDRYGIQTHLVAYHDHNGASARPKLLARLAAGERVALI
SDAGTPLVSDPGFKLVVEAAEAGFPVHPVPGASALLAALVAAGLPTDCFLFDGFLPPKAG
QRRNRISALAGVPGTLVFYETGPRLAESLADLADVLGPRPAAVCRELTKAFEEVRRADLT
ALAAHYADAEPPKGEIVLVIGPPLEEAAGDADVDAALIRALKDTSLKDAVAAVAGATGRP
KREVYARALAITQAKSGDADADA