Protein Info for GFF2242 in Xanthobacter sp. DMC5

Annotation: Carbamoyltransferase HypF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 769 PF00708: Acylphosphatase" amino acids 14 to 93 (80 residues), 66.1 bits, see alignment E=7e-22 TIGR00143: carbamoyltransferase HypF" amino acids 14 to 763 (750 residues), 766.8 bits, see alignment E=1.1e-234 PF07503: zf-HYPF" amino acids 112 to 145 (34 residues), 55.9 bits, see alignment (E = 7.6e-19) amino acids 162 to 194 (33 residues), 65.6 bits, see alignment (E = 7.1e-22) PF01300: Sua5_yciO_yrdC" amino acids 212 to 386 (175 residues), 134 bits, see alignment E=1e-42 PF17788: HypF_C" amino acids 403 to 502 (100 residues), 106.1 bits, see alignment E=3.4e-34 PF22521: HypF_C_2" amino acids 514 to 759 (246 residues), 252.2 bits, see alignment E=1.6e-78

Best Hits

KEGG orthology group: K04656, hydrogenase maturation protein HypF (inferred from 78% identity to xau:Xaut_0341)

Predicted SEED Role

"[NiFe] hydrogenase metallocenter assembly protein HypF" in subsystem NiFe hydrogenase maturation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (769 amino acids)

>GFF2242 Carbamoyltransferase HypF (Xanthobacter sp. DMC5)
MEAGEMDGALGETIEISGIVQGVGFRPFVYRLALRLGLDGTVSNAGGRVLIRAGGPRAAL
DAFAAALVAEAPPLARIERVERHPAALPEPGFRIVESGAGTVSIGVVPDVATCPACLAEI
ADPTTRRHRYAFTNCTDCGPRFSIVAGLPYDRPATTMAGFAMCPACRAEYENPADRRFHA
QPIACPACGPRLWLEREGAEIASTNPIHDAAELLRAGRILAVKGIGGFHIACDATDAGAV
ATLRTRKHRPTKPLAVMADLPTSHRLCVISPEEEAALLAPSAPILLLPLRPDAGLLPGLA
PGQSHLGLMLPYTPLHHLLIAGAGRPLVMTSGNRSSEPQIFRDDEARAGLAGIVDGFLMH
DRPIARRLDDSVARMAAGGLRVIRRGRGFAPLPLALPEDFEGAPPVLAMGGELKSALCLT
HGTRALLSHHLGDLDEPATGDAFETALADYSALFAHTPQVIAADLHPDYRASRLGAALAE
ARGLPLESVQHHHAHIAAAMAENGWRRADGKVVGIALDGLGMGEDGAVWGAEVLVCDYRE
SRRSARLSPIPLAGGDAASREPWRVLLAHLDRALGRAAVDGDAGLAALFAGKPVPTLRAM
MDKGLNAPLASSAGRLFDAVATLLGLAPDRLAHEGEAAMALEAVAEGEAAPYPFAISAQG
DLMEIDPAPLWRALLADIASGRARPTMAAAFHAGLADAFVSVAVQATEEAGIPAVALSGG
VFQNVRLLEETVARLRACGLTPLIPSHVPANDGGLAFGQAVVAAARQMA