Protein Info for GFF2234 in Sphingobium sp. HT1-2

Annotation: Adenylosuccinate lyase (EC 4.3.2.2) @ SAICAR lyase (EC 4.3.2.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00928: adenylosuccinate lyase
  • amino acids 2 to 435 (434 residues), 486.3 bits, E=4.1e-150
• PF00206: Lyase_1
  • amino acids 44 to 288 (245 residues), 167.4 bits, E=5.4e-53
• PF10397: ADSL_C
  • amino acids 354 to 434 (81 residues), 81.6 bits, E=4.1e-27

Best Hits

Swiss-Prot: 52% identical to PUR8_BACSU: Adenylosuccinate lyase (purB) from Bacillus subtilis (strain 168)

KEGG orthology group: K01756, adenylosuccinate lyase [EC: 4.3.2.2] (inferred from 95% identity to sjp:SJA_C1-12940)

MetaCyc: 36% identical to adenylosuccinate lyase (Methanocaldococcus jannaschii)
Adenylosuccinate lyase. [EC: 4.3.2.2]; 4.3.2.2 [EC: 4.3.2.2]; 4.3.2.- [EC: 4.3.2.2]

Predicted SEED Role

"Adenylosuccinate lyase (EC 4.3.2.2)" in subsystem De Novo Purine Biosynthesis or Purine conversions (EC 4.3.2.2)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (42/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (23/26 steps found)
• inosine-5'-phosphate biosynthesis I (6/6 steps found)
• inosine-5'-phosphate biosynthesis II (5/5 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis I (5/5 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis II (6/7 steps found)
• superpathway of purine nucleotide salvage (11/14 steps found)
• adenosine ribonucleotides de novo biosynthesis (3/3 steps found)
• inosine-5'-phosphate biosynthesis III (5/6 steps found)
• dZTP biosynthesis (4/5 steps found)
• tetrahydromethanopterin biosynthesis (3/14 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Biosynthesis of alkaloids derived from histidine and purine
• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.3.2.2

Protein Sequence (438 amino acids)

>GFF2234 Adenylosuccinate lyase (EC 4.3.2.2) @ SAICAR lyase (EC 4.3.2.2) (Sphingobium sp. HT1-2)
MVPRYSRPAMTALWEPEARFKIWFEIEAHATEKLGELGVVPPSAAKALWDWWATNPAIDV
PAIDAIEAVTKHDVIAFLTWVAEQVGDEARFMHQGMTSSDVLDTCLAVQLTRAADLLIED
LDKLLDVIKRRAFEHKLTPTIGRSHGIHAEPVTFGLKMAEAYAEFSRCKTRLVAAREEVA
TCAISGAVGTFANIDPRVEEHVAEKLGLAIEPVSTQVIPRDRHAMFFATLGVIASSIERL
AVEVRHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPVLTENLTGLARMVRSYALPAMENVA
LWHERDISHSSVERYIGPDATITLDFALGRLTGVIDKLLVYPERMMKNLDKMGGLVHSQR
VLLALTQAGVSREDSYRYVQRNAMKVWESDGQLSLLELLKADEDVAKALSAQEIEDKFNL
DYHFKQVDTIFARVFGEA