Protein Info for GFF2232 in Sphingobium sp. HT1-2

Annotation: Xylan alpha-1,2-glucuronosidase (EC 3.2.1.131)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 719 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details PF03648: Glyco_hydro_67N" amino acids 34 to 147 (114 residues), 56.1 bits, see alignment E=6.6e-19 PF07488: Glyco_hydro_67M" amino acids 153 to 470 (318 residues), 506.9 bits, see alignment E=3.5e-156 PF07477: Glyco_hydro_67C" amino acids 472 to 694 (223 residues), 307.8 bits, see alignment E=6.6e-96

Best Hits

Swiss-Prot: 56% identical to AGUA_CELJU: Extracellular xylan exo-alpha-(1->2)-glucuronosidase (gla67A) from Cellvibrio japonicus (strain Ueda107)

KEGG orthology group: K01235, alpha-glucuronidase [EC: 3.2.1.139] (inferred from 80% identity to sjp:SJA_C2-03470)

Predicted SEED Role

"Alpha-glucuronidase (EC 3.2.1.139)" in subsystem D-Galacturonate and D-Glucuronate Utilization (EC 3.2.1.139)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.139

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (719 amino acids)

>GFF2232 Xylan alpha-1,2-glucuronosidase (EC 3.2.1.131) (Sphingobium sp. HT1-2)
MFARLFCWIMASILLIGALLIGAPRALAEDGYDLWLRNAPADAAGAQRIGANARSIVGGA
SPTLMIARRELQRGIAGLTGTTVPLTATVQQGAILLQSAAQQDKPSVPTDGLGADGYAIR
SLTIAGKPVTVIAGATDVGVLHGAYAWLRLARTGAALDRIDMRSAPRIGLRLLNHWDNLD
GTVERGYAGQSIWDWWRLPDYKGPRYTDYARANASLGINGTVLNNVNAKSDSLTAAYIAK
AAALADLFRPYGIKVYLSVKWTAPMELDGLKSADPLDPAVAAWWKAKADEIYKAIPDFGG
FLVKANSEGQPGPQDYKRTHADGANMLAAALKPHGGIVMWRAFVYAHDNPDDRAKQAYSD
FKPLDGQFADNVIVQVKNGAIDFQPREPFHPLFGAMPKTPLMMEFQITKEYLGQSTHLTY
LGTLFEETLKSDTLARGKGSTVAKVIDGSLEGHKLTGIAGVANIGVDRDWSGSIFNQADW
YAFGRMAWDTDLTAEAVAREWAAQTFSPDPRVVAPIVAMMMGSREAAVDYMTPLGLAHIM
GTGHHYGPAPWVSELARPEWNPVYYHKADRQGIGFDRTRTGSNATGQYAPALAKLLDNPK
TTPERELLWFHHLPWDYRLASGETLWDGLIHHYDRGVETVAAMQRDWAKLKPQVDAERFA
QVETFLAIQHREAQWWRDACISYFQSVSGRSLPVGSAAPPHPLDWYRALSFPYAPGHPK