Protein Info for GFF2227 in Sphingobium sp. HT1-2

Annotation: D-mannonate oxidoreductase (EC 1.1.1.57)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 486 PF01232: Mannitol_dh" amino acids 29 to 273 (245 residues), 160.6 bits, see alignment E=4.9e-51 PF08125: Mannitol_dh_C" amino acids 284 to 466 (183 residues), 155.7 bits, see alignment E=1.1e-49

Best Hits

Swiss-Prot: 44% identical to YEIQ_ECOLI: Uncharacterized oxidoreductase YeiQ (yeiQ) from Escherichia coli (strain K12)

KEGG orthology group: K00040, fructuronate reductase [EC: 1.1.1.57] (inferred from 82% identity to sjp:SJA_C2-03410)

Predicted SEED Role

"D-mannonate oxidoreductase (EC 1.1.1.57)" in subsystem D-Galacturonate and D-Glucuronate Utilization (EC 1.1.1.57)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.57

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (486 amino acids)

>GFF2227 D-mannonate oxidoreductase (EC 1.1.1.57) (Sphingobium sp. HT1-2)
VTRLSSATLAGLPADIVRPGYDRAAVRPGVVHLGIGAFNRAHQAVIFDDALTAGDLRWGV
IAASLRSPGVRDQMMPQDCLYTMLVRDGSAEQARIIGVVSEVLVAPEDPAALVAALAAPD
THIVTLTVTEKGYKLDPATGALIEGDPQLAADLASLAAPQTAPGFLVAALAARRAAGLPP
FTAISCDNLPHNGTRLRNAVLALAARHDPALADWIAAEGAFPETMVDRIVPATTDADIAA
LAARLGVEDQAMVKAEPFLQWVIEDKFCGPRPDFGAGVQVTAAVAPWEEAKLRLLNGAHS
GIAYLGGLAGIDHVHEVLALPEARHFVETLWDEAQTTLSPPPELDVAAYRRELMGRFDNP
TLQHRTRQIAMDGSQKLPQRLLATIAARLSAGQGIDALSLAVAAWVRWQAGVDDRGEAHV
VDDPLAAAIAARLADAPSTEARVDAILAFDAVVPADLAGNAAFRAALIRWLAILEADGAR
AALAQL