Protein Info for GFF2226 in Sphingobium sp. HT1-2

Annotation: beta-galactosidase (EC 3.2.1.23)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 893 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF02837: Glyco_hydro_2_N" amino acids 127 to 247 (121 residues), 50.3 bits, see alignment E=8.6e-17 PF22666: Glyco_hydro_2_N2" amino acids 158 to 231 (74 residues), 29 bits, see alignment E=3.1e-10 PF00703: Glyco_hydro_2" amino acids 262 to 372 (111 residues), 49.2 bits, see alignment E=2.6e-16 PF02836: Glyco_hydro_2_C" amino acids 379 to 585 (207 residues), 101.3 bits, see alignment E=1.8e-32 PF16355: DUF4982" amino acids 710 to 769 (60 residues), 77.2 bits, see alignment 2.2e-25 PF18565: Glyco_hydro2_C5" amino acids 782 to 883 (102 residues), 121.3 bits, see alignment E=4.6e-39

Best Hits

KEGG orthology group: K01190, beta-galactosidase [EC: 3.2.1.23] (inferred from 65% identity to aex:Astex_3600)

Predicted SEED Role

"Beta-galactosidase (EC 3.2.1.23)" in subsystem Galactosylceramide and Sulfatide metabolism or Lactose and Galactose Uptake and Utilization or Lactose utilization (EC 3.2.1.23)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.23

Use Curated BLAST to search for 3.2.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (893 amino acids)

>GFF2226 beta-galactosidase (EC 3.2.1.23) (Sphingobium sp. HT1-2)
MKTLRTILLASCLLSPATLLAAPRDSVSISRDWRFTKGDPAGLTEDLRYDVRPRIEDAGD
GKVADARPDAAVQVDDSGARVLKPWILPSANPFIADPAKHHTRPAGNPGGTVSYVQPGFD
DSGWTHVDLPHDWAIAGPFIKDGPYGGMGRLPSWGIGWYRKALTIPASDKGKSIFLDVEG
AMSYATVWLNGRLVGGWPYGYNSFRLDLTPYAVPGGQNQLVIRLDNPQASARWYPGGGLY
RDIYLTTTNKVHVGQWGSIVRTPQVSKAQASVDLSLTLDNDGDTSAQVEVATALYAIDAS
GKRIGRPVASIARQSTSIAPHGKAELKGSTILSNPRLWGPPPTQAPNRYVAVSTVTQGGK
LIDSYETRFGVRDIRFDPDKGVIVNGEQIPLRGVNNHHDLGAIGAAFNARAAERQLEILR
DMGTNAVRMSHNPPAPELLELTDRMGFLVMDEVFDSWEKKKTPHDFHLIFPDWHEADARA
MLRRDRNHPSIIIWSIGNEVGEQYDGEAGAKIGRELVAIAHQEDPTRPATSAMNYAKADM
LLPTTVDVISLNYQGAGIRGIVGQYPAFRAKFPDKVILSTESASALSSRGEYMFPVAGAI
SGPVRPWSGGNPDTHQVSAYEMHAADFGSSPDRVWAADDQNPYVAGEFVWTGFDYLGEPT
PYYTSRSSYSGIVDLAGFPKDRFWLYQARWRPDLKFAHILPHWTWPDRVGQITPVHVFSS
ADEAELFVNGKSQGKVKKRDYEYRFRWDYVVYEPGEVKVVTWKKGQPWATETIRTVGEAA
KLSLTADRSAIADDGRDLSFVTLKILDKDGNVVPAAKQDIRFSIEGPGEIIATDNGDPTD
LTAFPSHDRKAFNGLALVIVKAKPGEKGRVTVRASAPGLTAAQAVISTNATVR